製品の概要

  • 製品名Anti-STAT3 (phospho S727) antibody
    STAT3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to STAT3 (phospho S727)
  • 特異性ab30647 detects endogenous levels of STAT3 only when phosphorylated at Serine 727.
  • アプリケーション適用あり: IHC-FoFr, IHC-P, WB, ELISA, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to Human STAT3 (phospho S727). The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P-R).
    Database link: P40763
    (Peptide available as ab43618, ab82053)

  • ポジティブ・コントロール
    • IHC: breast carcinoma WB: HeLa cells lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 20950279
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).Can be blocked with Recombinant Human STAT3 protein (ab43618).
ELISA 1/10000.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
  • 組織特異性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 関連疾患Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
    Autoimmune disease, multisystem, infantile-onset
  • 配列類似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻訳後修飾Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
  • 細胞内局在Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
  • Information by UniProt
  • 参照データベース
  • 別名
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    • Signal transducer and activator of transcription 3 (acute phase response factor) antibody
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Anti-STAT3 (phospho S727) antibody 画像

  • All lanes : Anti-STAT3 (phospho S727) antibody (ab30647)

    Lane 1 : HeLa cells extract
    Lane 2 : HeLa cells extract with Synthesized non-phosphopeptide
    Lane 3 : HeLa cells extract with Synthesized phosphopeptide


    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • ab30647 at 1/50 dilution staining STAT3 (phospho S727) in human breast carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). The left image show staining by ab30647 without specific peptide while right image show staining after blocking with peptide.

Anti-STAT3 (phospho S727) antibody (ab30647) 使用論文

This product has been referenced in:
  • Cui L  et al. Downregulation of B7-H4 in the MHCC97-H hepatocellular carcinoma cell line by arsenic trioxide. Mol Med Rep 13:2032-8 (2016). WB ; Human . Read more (PubMed: 26781180) »
  • Zhang L  et al. STAT3 is a key pathway in primary intraosseous squamous cell carcinoma arising from an odontogenic keratocyst. Am J Transl Res 7:1860-9 (2015). IHC-P ; Human . Read more (PubMed: 26692930) »

See all 12 Publications for this product

Product Wall

Application Western blot
Loading amount 45 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Lung)
Specification Lung
Treatment Basel conditions
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

投稿 Oct 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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Dr. Ruma Raha-Chowdhury

Verified customer

投稿 May 11 2012

Thank you for your inquiry.

I strongly suggest to use a pre-absorbed secondary antibody so that the cross-binding to the two rat monoclonals can be minimized.

Since you are already using fluorescence in the green and red spectrum,...

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