製品の概要
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製品名Anti-STAT2 antibody [Y141]
STAT2 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [Y141] to STAT2
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由来種Rabbit
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特異性This antibody detects both long and short forms of STAT2.
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アプリケーション適用あり: WB, ICC, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide within Human STAT2 (N terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- K562 cell lysate and human thyroid cancer
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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Concentration information loading... -
精製度IgG fraction
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ポリ/モノモノクローナル
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クローン名Y141
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アイソタイプIgG
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研究分野
関連製品
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab32367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/5000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). | |
| ICC | 1/50 - 1/100. | |
| IHC-P | 1/100 - 1/250. |
ターゲット情報
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機能Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
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配列類似性Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻訳後修飾Tyrosine phosphorylated in response to IFN-alpha.
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細胞内局在Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
- Information by UniProt
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参照データベース
- Entrez Gene: 6773 Human
- Entrez Gene: 20847 Mouse
- Entrez Gene: 288774 Rat
- Omim: 600556 Human
- SwissProt: P52630 Human
- SwissProt: Q9WVL2 Mouse
- Unigene: 530595 Human
- Unigene: 293120 Mouse
see all -
別名
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF 3 antibody
see all
画像
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![Western blot - Anti-STAT2 antibody [Y141] (ab32367)](http://a.abcam.com/ps/products/32/ab32367/Images/ab32367-298807-ab32367-KO-WB-EWAR.jpg)
Western blot - Anti-STAT2 antibody [Y141] (ab32367)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: STAT2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: K562 whole cell lysate (20 µg)
Lane 4: THP1 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab32367 was shown to recognize STAT2 in wild-type HAP1 cells as signal was lost at the expected MW in STAT2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and STAT2 knockout samples were subjected to SDS-PAGE. Ab32367 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-STAT2 antibody [Y141] (ab32367)](http://a.abcam.com/ps/products/32/ab32367/Images/ab32367_1.jpg)
Western blot - Anti-STAT2 antibody [Y141] (ab32367)Anti-STAT2 antibody [Y141] (ab32367) at 1/500 dilution + K562 cell lysate
Predicted band size: 98 kDa
Observed band size: 100 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)](http://a.abcam.com/ps/products/32/ab32367/Images/ab32367_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)Immunohistochemical analysis of paraffin-embedded human thyroid cancer using ab32367
参考文献
ab32367 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"