This antibody was purified on a phosphopeptide bound column using immunoaffinity chromatography. It was also cross-absorbed against the non-phosphorylated peptide to remove antibodies that may detect the non-phosphorylated form of the protein.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB (ECL): Use at a concentration of 1 µg/ml. Detects a band of approximately 90 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Belongs to the transcription factor STAT family. Contains 1 SH2 domain.
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.