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Abcam's Starch Assay Kit provides an easy, accurate assay to measure starch levels in a variety of samples. In the assay, starch is hydrolyzed to glucose which is oxidized to generate color (570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay can detect starch at 0.0004 to 2 mg/ml.
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Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear a-1,4 linked polymer) and amylopectin, (highly a-1,6 branched a-1,4 polymer). Starch generally contains 0-25% amylose and 75-100% amylopectin.
|Development Buffer||WM||1 x 25ml|
|Development Enzyme Mix (Lyophilised)||Green||1 vial|
|Hydrolysis Buffer||NM||1 x 25ml|
|Hydrolysis Enzyme Mix (Lyophilised)||Blue||1 vial|
|OxiRed Probe in DMSO||Red||1 vial|
|Starch Standard (2.0 mg/ml)||Yellow||1 x 100µl|
Our Abpromise guarantee covers the use of ab83393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|