The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 10 µg/ml.
At 100µl/well, the following results are obtained:
10 ng/ml SEA Abs (410 nm) = 2.00 after 15 minute incubation.
1.25 ng/ml SEA Abs (410 nm) = 0.78 after 15 minute incubation.
WB: Use at a concentration of 0.1 µg/ml. Predicted molecular weight: 28 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This secreted toxin is responsible for the symptoms of toxic shock syndrome. It belongs to the staphylococcal/streptococcal toxin family.
Baroja ML et al. The SaeRS Two-Component System Is a Direct and Dominant Transcriptional Activator of Toxic Shock Syndrome Toxin 1 in Staphylococcus aureus. J Bacteriol198:2732-42 (2016).
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