製品の概要

  • 製品名Anti-Staphylococcus aureus antibody
    Staphylococcus aureus 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Staphylococcus aureus
  • 特異性Soluble and structural antigens of the whole bacterium. Antiserum is not absorbed for related microorganisms.
  • アプリケーション適用あり: ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Staphylococcus aureus
  • 免疫原

    Staphylococcus aureus strain ATCC 27660 (mixture of intact organisms and lysed cells).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab20920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Use with acetone fixed tissues.

ターゲット情報

  • 関連性Staphylococcus aureus are gram-positive bacteria that, although part of the normal flora of humans (nasal passages, skin and mucous membranes), have the potential to become opportunistic pathogens. S. aureus is a frequent cause of a wide range of infections and some bacterial strains have become resistant to many commonly used antibiotics.
  • 別名
    • S. aureus antibody

Anti-Staphylococcus aureus antibody 画像

  • ab20920 staining an abscess in rat calf tissue prepared as a 20 µm cryoslice.  Tissue was fixed in paraformaldehyde, permeabilised with 0,5% Trition X100 in PBS, and blocked with 10% serum plus 1% BSA for 1 hour at room temperature.  The primary antibody was diluted 1/500 with PBS plus 3-5% Serum, 0,5% Trition X100 and incubated for 24 hours at 20°C.  An Alexa Fluor® 568 goat anti-rabbit was used as the secondary

    See Abreview

  • ab20920 staining Staphylococcus aureus in human keratinocytes by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in methanol, permeabilized using 0.1% saponin/ PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab20920 at 10µg/ml for 14 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

    Red: cadherin
    Blue: DAPI
    Green: anti-Staphylococcus aureus (ab20920)

    See Abreview

Anti-Staphylococcus aureus antibody (ab20920) 使用論文

This product has been referenced in:
  • Waryah CB  et al. An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus. Folia Microbiol (Praha) 61:473-478 (2016). Read more (PubMed: 27106696) »
  • Hamza T & Li B Differential responses of osteoblasts and macrophages upon Staphylococcus aureus infection. BMC Microbiol 14:207 (2014). Read more (PubMed: 25059520) »

See all 7 Publications for this product

Product Wall

The Hamza and Li publication BMC Microbiol 14:207 (2014) cites ab20920 as detecting the ATCC 25923 strain so there is evidence it will cross react.

The cells used for the immunogen started out as whole cells that were disrupted by sonication and freeze/thaw cycles. Therefore the immunogen is a mixture of intact Staph aureus organisms and soluble proteins from disrupted cells. This is no record of ...

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Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample Human Tissue sections (skin (from atopic patient))
Specification skin (from atopic patient)
Permeabilization Yes - 0,1% Saponin / PBS
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Jul 17 2013

Thank you very much for your email.
There is absolutely no problem, the antibody will still work properly in my opinion.
Indeed, we have tested different antibodies, and even after a week at 37 degrees Celcius, the antibodies did not seem to ...

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Thank you for contacting us.

Please find below the information I managed to collect by contacting the sources of the Anti-Staphylococcus aureusand the Anti-Streptococcus agalactiaeantibodies we have in our catalog :

Anti-Staphyloco...

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Application Immunoprecipitation
Sample Staphylococcus aureus Cell lysate - whole cell (Primmary Osteoblasts cells)
Total protein in input 200000 cells
Specification Primmary Osteoblasts cells
Immuno-precipitation step Other - Dynabeads 280 (invitrogen)
Username

Dr. Lorena Tuchscherr

Verified customer

投稿 Feb 23 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Keratinocytes)
Specification Keratinocytes
Fixative Methanol
Permeabilization Yes - 0,1% Saponin/PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 20 2012

Thank you for contacting us. Indeed, neither ab68954 nor ab20920 have been tested for immunoprecipitation (IP) yet, but they could theoretically be used in this application. If it is just between the two, I would recommend ab20920 because it is an u...

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Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (abscess in rat calf - cryoslice)
Specification abscess in rat calf - cryoslice
Fixative Paraformaldehyde
Permeabilization Yes - 0,5% Trition X100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Dagmar Salber

Verified customer

投稿 Jan 09 2008

Application Immunocytochemistry
Sample Staphylococcus aureus Cultured Cells (Human keratinocytes)
Specification Human keratinocytes
Fixative No fixative - LIVE cells
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Jul 26 2007

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"