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Staphylococcus aureus strain ATCC 27660 (mixture of intact organisms and lysed cells).
Our Abpromise guarantee covers the use of ab20920 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. Use with acetone fixed tissues.|
ab20920 staining an abscess in rat calf tissue prepared as a 20 µm cryoslice. Tissue was fixed in paraformaldehyde, permeabilised with 0,5% Trition X100 in PBS, and blocked with 10% serum plus 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/500 with PBS plus 3-5% Serum, 0,5% Trition X100 and incubated for 24 hours at 20°C. An Alexa Fluor® 568 goat anti-rabbit was used as the secondary