Anti-Staphylococcus alpha Hemolysin 抗体 (Biotin) (ab15950)

製品の概要

  • 製品名
    Anti-Staphylococcus alpha Hemolysin antibody (Biotin)
    Staphylococcus alpha Hemolysin 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to Staphylococcus alpha Hemolysin (Biotin)
  • 標識
    Biotin
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Staphylococcus aureus
  • 免疫原

    Highly pure (> 95%) Staphylococcus aureus alpha Hemolysin alpha

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/2000. This dilution of antibody will give an absorbance of > 1.0 for a 10ng standard after a 5 minute incubation.
WB 1/1000. Predicted molecular weight: 35.9 kDa.

ターゲット情報

  • 関連性
    Staphylococcus aureus alpha Hemolysin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. The toxin self-assembles to form first, a nonlytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure. Heptamer oligomerization and pore formation is required for lytic activity.
  • 細胞内局在
    Secreted
  • 参照データベース
    • 別名
      • Alpha hemolysin antibody
      • Alpha HL antibody
      • Alpha toxin antibody
      • Hla antibody
      • Hly antibody
      • Staphylococcus alpha HL antibody
      • Staphylococcus alpha toxin antibody
      see all

    Anti-Staphylococcus alpha Hemolysin antibody (Biotin) 画像

    • All lanes : Anti-Staphylococcus alpha Hemolysin antibody (Biotin) (ab15950) at 1/1000 dilution

      Lane 1 : Positive control (purified Hla (100ng/ml))
      Lane 2 : Staph. aureus infected skin from patient with atopic dermatitis - untreated
      Lane 3 : Staph. aureus infected skin from patient with atopic dermatitis - treated with HBD2 (0,2µg/ml)
      Lane 4 : Staph. aureus infected skin from patient with atopic dermatitis - treated with HBD2 (2,0µg/ml)
      Lane 5 : Staph. aureus infected skin from patient with atopic dermatitis - treated with HBD2 (20µg/ml)
      Lane 6 : Staph. aureus infected skin from patient with atopic dermatitis - treated with penicillin (50ng/ml)

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 35.9 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

      Image courtesy of an anonymous Abreview.

      See Abreview

    プロトコール

    Anti-Staphylococcus alpha Hemolysin antibody (Biotin) (ab15950) 使用論文

    ab15950 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    ELISA
    Sample
    Staphylococcus aureus Cell (different dilutions of S. aureus aplha toxin)
    Specification
    different dilutions of S. aureus aplha toxin
    Type
    Sandwich (Detection)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 22 2013

    I can understand that you are disappointed. I am sorry for this wrong information that was cited on the datasheet. I am pleased to provide another vial of the ab15950 for you.


    I have issued a free of charge replacement with the order numb...

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    Thank you very much for your patience. I am sorry that you experiencing problems with this two antibodies.

    I am still waiting for some QC results from the lab and I will forward this to you as soon as I recive the answer.

    Every appli...

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    Thank you for contacting us.

    In order to help you with these antibodies I would like to get some more information about the experiment.

    1. Do I understand it right that the performed experiment is a sandwich ELISA? Do you mean the ab1...

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    Thank you for contacting us. I have spoken with the lab regarding your questions.

    I am sorry to say I have no definite answers to any of these questions. For the third question, I have a probable answer; as the antiserum is a polyclonal the ...

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    Thank you for your response.

    Many of the questions seem to be about the alpha Hemolysin protein.Just to be sure, I would like clarify that this product, ab15950 Anti-Staphylococcus alpha Hemolysin antibody (Biotin), is an antibody raised in ...

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    Thank you for contacting us.

    The productab15950 Anti-Staphylococcus alpha Hemolysin antibody (Biotin) has been conjugated with biotin and will be recognized by streptavidin.

    I hope this information is helpful to you. Please do...

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    Application
    Western blot
    Sample
    Human Tissue lysate - whole (skin biopsie)
    Loading amount
    15 µg
    Specification
    skin biopsie
    Treatment
    see below
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16% gel)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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