製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to STAM2
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human STAM2 around the phosphorylation site of tyrosine 192 (S-L-YP-P-S).

  • ポジティブ・コントロール
    • NIH/3T3 cell extracts treated with EGF (200ng/ml, 30mins).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab63372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

ターゲット情報

  • 機能
    Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the STAM family.
    Contains 1 ITAM domain.
    Contains 1 SH3 domain.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • ドメイン
    The VHS and UIM domains mediate the interaction with ubiquitinated proteins.
    The SH3 domain mediates the interaction with USP8.
    Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • 翻訳後修飾
    Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.
  • 細胞内局在
    Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp564C047 antibody
    • Hbp antibody
    • Hrs-binding protein antibody
    • HSE1 homolog antibody
    • Signal transducing adapter molecule 2 antibody
    • Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 antibody
    • Signal transducing adaptor molecule 2A antibody
    • Signal transducing adaptor molecule 2B antibody
    • Signal transducing adaptor molecule antibody
    • STAM like protein containing SH3 and ITAM domains 2 antibody
    • STAM-2 antibody
    • Stam2 antibody
    • STAM2_HUMAN antibody
    • STAM2A antibody
    • STAM2B antibody
    see all

画像

  • All lanes : Anti-STAM2 antibody (ab63372) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extracts treated with EGF (200ng/ml, 30mins)
    Lane 2 : NIH/3T3 cell extracts treated with EGF (200ng/ml, 30mins) with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 38 kDa,>117 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

This product has been referenced in:
  • Kapuralin K  et al. STAM2, a member of the endosome-associated complex ESCRT-0 is highly expressed in neurons. Mol Cell Neurosci 67:104-15 (2015). WB, IHC-FoFr, ICC/IF ; Mouse . Read more (PubMed: 26101075) »
  • Curlin M  et al. Stam2 expression pattern during embryo development. Gene Expr Patterns 12:68-76 (2012). WB, IHC ; Mouse . Read more (PubMed: 22143071) »

See all 2 Publications for this product

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