The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
追加情報Is unsuitable for WB.
機能Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
Detection of Human STAM1 in Immunoprecipitates of Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells using ab117817 at 6 µg/mg of lysate. Subsequent WB detection was done using an other anti Human STAM1 antibody.
Detection: Chemiluminescence with an exposure
time of 10 seconds.
Anti-STAM1 antibody (ab117817) 使用論文
has not yet been referenced specifically in any publications.