製品の概要

  • 製品名Anti-STAM1 antibody
    STAM1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to STAM1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 300-350 of Human STAM1 according to NP_003464.1.

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa, 293T and Jurkat cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab117813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Belongs to the STAM family.
    Contains 1 ITAM domain.
    Contains 1 SH3 domain.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • 翻訳後修飾Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB.
  • 細胞内局在Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HSE1 homolog antibody
    • Signal transducing adapter molecule 1 antibody
    • signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 antibody
    • STAM antibody
    • STAM-1 antibody
    • STAM1_HUMAN antibody
    see all

Anti-STAM1 antibody 画像

  • All lanes : Anti-STAM1 antibody (ab117813) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 50 µg
    Lane 4 : Whole cell lysate from Jurkat cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 58 kDa


    Exposure time : 30 seconds
  • ab117813 at 1 µg/ml staining STAM1 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
    Lane 1; IP using ab117813 at 6 µg/mg lysate.
    Lane 1; IP using an antibody to a downstream epitope.
    Lane 2; IP using an antibody to an alternative downstream epitope.
    Lane 3; IP using control IgG.
    1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 10 second exposure.

Anti-STAM1 antibody (ab117813) 使用論文

ab117813 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"