Anti-ST6GALNAC5 抗体 (ab69855)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ST6GALNAC5
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog, Pig, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide: KGQVYNNLHL LSQVLPRLKA FMITRHKMLQ FDELFKQETG KDRKISNTWL, corresponding to amino acids 182-231 of Human ST6GALNAC5

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab69855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Involved in the biosynthesis of ganglioside GD1a from GM1b. It exhibits higher activity with glycolipids than with glycoproteins.
  • パスウェイ
    Protein modification; protein glycosylation.
  • 配列類似性
    Belongs to the glycosyltransferase 29 family.
  • 細胞内局在
    Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6-sialyltransferase 5 antibody
    • 6-sialyltransferase V antibody
    • Alpha N acetylgalactosaminide alpha 2,6 sialyltransferase 5 antibody
    • Alpha-N-acetylgalactosaminide alpha-2 antibody
    • GalNAc alpha 2,6 sialyltransferase V antibody
    • GalNAc alpha-2 antibody
    • GD1 alpha synthase antibody
    • SIA7E_HUMAN antibody
    • Sialyltransferase 7E antibody
    • Sialyltransferase E antibody
    • SIAT7 E antibody
    • SIAT7-E antibody
    • SIAT7E antibody
    • ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 antibody
    • ST6GalNAc V antibody
    • St6galnac5 antibody
    • ST6GalNAcV antibody
    see all

Anti-ST6GALNAC5 antibody 画像

  • Anti-ST6GALNAC5 antibody (ab69855) at 1.25 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Anti-ST6GALNAC5 antibody (ab69855) at 1/5000 dilution (in PBS + 0.1% Tween + 5% milk powder for 16 hours at 4°C) + Whole cell lysate of Human WT MDA MB231 cells at 20 µg

    Secondary
    An HRP-polymer Goat anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

プロトコール

Anti-ST6GALNAC5 antibody (ab69855) 使用論文

ab69855 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email and the additional information and the image.
From the image, it seems that either too much protein, too much primary or too much secondary antibody is being used or the blot is very overexposed, as the bands are too dark ...

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Thank you for contacting us.
I am sorry to hear you are experiencing problems with our antibody.
Could you please also let me know the following details:
1) Where did you see the bands (you can also attach a WB image)?
2) What was t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (WT MDA MB231)
Loading amount
20 µg
Specification
WT MDA MB231
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Sep 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Lewis lung carcinoma)
Loading amount
10 µg
Specification
Lewis lung carcinoma
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Aug 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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