製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ST2
  • 特異性
    ab25877 reacts all three known ST2 isoforms.
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to 16 amino acids at the amino terminus of Mouse ST2.

  • ポジティブ・コントロール
    • Mouse kidney cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa.

ターゲット情報

  • 機能
    Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.
  • 組織特異性
    Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle.
  • 配列類似性
    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DER-4 antibody
    • DER4 antibody
    • FIT 1 antibody
    • Growth stimulation expressed antibody
    • homolog of mouse growth stimulation-expressed antibody
    • Il1rl1 antibody
    • IL33R antibody
    • ILRL1_HUMAN antibody
    • Interleukin 1 receptor like 1 antibody
    • interleukin 1 receptor related protein antibody
    • Interleukin-1 receptor-like 1 antibody
    • Protein ST2 antibody
    • ST2 antibody
    • ST2L antibody
    • ST2V antibody
    • T1 antibody
    • T1 protein antibody
    see all

画像

  • All lanes : Anti-ST2 antibody (ab25877) at 1 µg/ml

    Lane 1 : Mouse kidney lysate in presence of blocking peptide (1ug/ml)
    Lane 2 : Mouse kidney lysate in absence of blocking peptide


    Predicted band size : 40 kDa
  • Human lung cancer tissue stained for ST2 using ab25877 at 5 μg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human lung cancer tissue labeling ST2 with Anti-ST2 antibody (ab25877) at 5μg/ml.
  • Immunohistochemistry of ST2 in human liver tissue with ST2 antibody at 5 µg/ml.

  • ab25877 at 2µg/ml staining ST2 in mouse kidney tissue by IHC

  • Mouse kidney cells stained for ST2 using ab25877 at 20 μg/ml in ICC/IF.

参考文献

This product has been referenced in:
  • Chen M  et al. Evaluation of Anti-Inflammatory Activities of Qingre-Qushi Recipe (QRQS) against Atopic Dermatitis: Potential Mechanism of Inhibition of IL-33/ST2 Signal Transduction. Evid Based Complement Alternat Med 2017:2489842 (2017). Read more (PubMed: 28751921) »
  • O'Donnell C  et al. An antitumorigenic role for the IL-33 receptor, ST2L, in colon cancer. Br J Cancer 114:37-43 (2016). Read more (PubMed: 26679377) »

See all 8 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Ventana Protease 1
Permeabilization
No
Specification
Lung
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 28 2016

For staining formalin-fixed, paraffin-embedded tissue with the ST2 antibody ab25877, we recommend a heat-mediated antigen retrieval with 10mM sodium citrate, pH 5.5, at 95-100C. The optimal time of incubation will need to be determined for your samples...

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Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolée d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab25877

Notre poli...

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Je suis désolée d'apprendre qu’ab25877 ne vous donne pas de bons résultats en Western blot.

L’anticorps ab25877 devrait effectivement reconnaitre les 2 isoformes de la protéine à 38.5 kDa et 64.8...

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Thank you for contacting us and your interest in our products.

We only have one antibody which has been tested (and would be covered by the Abpromise guarantee) for IHC. This is the rabbit polyclonal http://www.abcam.com/ST2-antibody-ab25877....

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Fixative
Formaldehyde
Antigen retrieval step
Other
Permeabilization
No
Blocking step
R&D systems kit as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Dec 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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