製品の概要

  • 製品名Anti-ST18 antibody
    ST18 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ST18
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 900-949 (IKKGKVSEEL MTIKLKATGG IESDEEIRHL DEEIKELNES NLKIEADMMK) of Human ST18 (NP_055497)

  • ポジティブ・コントロール
    • Hela cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab86563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 115 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells.
  • 組織特異性Detected at low levels in heart, liver, kidney, skeletal muscle, pancreas, testis, ovary and prostate. Detected at even lower levels in mammary epithelial cells and breast cancer cells.
  • 配列類似性Contains 6 C2HC-type zinc fingers.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA0535 antibody
    • St18 antibody
    • ST18_HUMAN antibody
    • suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein) antibody
    • Suppression of tumorigenicity 18 protein antibody
    • Suppression of tumorigenicity protein 18 antibody
    • Zinc finger protein 387 antibody
    • Znf387 antibody
    see all

Anti-ST18 antibody 画像

  • Anti-ST18 antibody (ab86563) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 115 kDa
    Gel concentration: 6-18%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cerebellum tissue labelling ST18 with ab86563 at 5.0µg/ml.


  • Developed using the ECL technique

    Predicted band size : 115 kDa


    Exposure time : 1 minute

    Image courtesy of Stephan Bartholomä by Abreview.

    ab86563 used in Western Blot at a 1/1000 dilution. Whole cell lysate was prepared from leukemic cell lines and 5µg was added to each lane. A goat anti-rabbit polyclonal was used at a 1/10,000 dilution.

Anti-ST18 antibody (ab86563) 使用論文

ab86563 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Acute myeloide leukemic cell line KG-1)
Specification Acute myeloide leukemic cell line KG-1
Fixative Formaldehyde
Permeabilization Yes - Triton X 0,25%
Blocking step PBS with FCS (10%) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Mr. Stephan Bartholomä

Verified customer

投稿 Jul 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (leukemic cell lines)
Loading amount 5 µg
Specification leukemic cell lines
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 20°C
Username

Mr. Stephan Bartholomä

Verified customer

投稿 Apr 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"