Customer reviews and FAQs

製品の概要

  • 製品名Anti-ST14 antibody - Catalytic domain
    ST14 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ST14 - Catalytic domain
  • 特異性This antibody does not recognize matriptase 2 (MTSP2).
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide based on the catalytic domain of Human ST14.

    Immunogen の所有権に関して

    (Peptide available as ab41264.)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent dilution. Predicted molecular weight: 95 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates.

ターゲット情報

  • 機能Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site.
  • 関連疾患Defects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH) [MIM:610765]. ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair.
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 4 LDL-receptor class A domains.
    Contains 1 peptidase S1 domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Epithin antibody
    • HAI antibody
    • Matriptase antibody
    • Membrane type serine protease 1 antibody
    • Membrane type serine protease antibody
    • Membrane-type serine protease 1 antibody
    • MT SP1 antibody
    • MT-SP1 antibody
    • MTSP 1 antibody
    • MTSP1 antibody
    • Prostamin antibody
    • PRSS 14 antibody
    • PRSS14 antibody
    • Serine protease 14 antibody
    • Serine protease TADG 15 antibody
    • Serine protease TADG-15 antibody
    • Serine protease TADG15 antibody
    • SNC 19 antibody
    • SNC19 antibody
    • ST 14 antibody
    • ST14 antibody
    • ST14_HUMAN antibody
    • Suppression of tumorigenicity 14 (colon carcinoma matriptase epithin) antibody
    • Suppression of tumorigenicity 14 (colon carcinoma) antibody
    • Suppression of tumorigenicity 14 antibody
    • Suppressor of tumorigenicity 14 protein antibody
    • Suppressor of tumorigenicity protein 14 antibody
    • TADG 15 antibody
    • TADG15 antibody
    • TMPRSS14 antibody
    • Tumor associated differentially expressed gene 15 protein antibody
    • Tumor-associated differentially-expressed gene 15 protein antibody
    see all

Anti-ST14 antibody - Catalytic domain 画像

  • Immunocytochemistry/ Immunofluorescence-ST14 antibody - Catalytic domain(ab28266)
    Immunocytochemistry/ Immunofluorescence-ST14 antibody - Catalytic domain(ab28266)
    ICC/IF image of ab28266 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28266, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

プロトコール

Anti-ST14 antibody - Catalytic domain (ab28266) 使用論文

This product has been referenced in:
  • Sun P  et al. Decreasing the ratio of matriptase/HAI-1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer. Mol Med Rep 14:1465-74 (2016). WB ; Human . Read more (PubMed: 27356668) »

See 1 Publication for this product

ab28266 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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ab28266 の abreview を投稿する ab28266 に関する問い合わせをする

1 Abreview or Q&A

10
Votes: Highest First

Customer kindly asked if we can provide any data relating to Matriptase 2 non specificfity.

Thank you for contacting us. After having discussion with my lab colleagues, regrettably I have to say we have not tested the cross reactivity of these antibodies with Matriptase 2. We do not have any data for sharing. The matriptase 2 non reac...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"