Anti-SSX1 抗体 [1E10] (ab119036)

製品の概要

  • 製品名Anti-SSX1 antibody [1E10]
    SSX1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1E10] to SSX1
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human SSX1 produced in E.coli (NP_005626).

  • ポジティブ・コントロール
    • HEK293T cell lysate transfected with pCMV6-ENTRY SSX1 cDNA; COS7 cells transiently transfected with pCMV6-ENTRY SSX1; HEK293T cells transfected with a SSX1 overexpress plasmid; Hela cells; Jurkat cells
  • 特記事項Dilute in PBS (pH7.3) before use.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)ab119036 was purified from mouse ascites fluids by affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名1E10
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab119036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 22 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

ターゲット情報

  • 機能Could act as a modulator of transcription.
  • 組織特異性Expressed at high level in the testis. Expressed at low level in thyroid. Not detected in tonsil, colon, lung, spleen, prostate, kidney, striated and smooth muscles. Detected in rhabdomyosarcoma and fibrosarcoma cell lines. Not detected in mesenchymal and epithelial cell lines.
  • 関連疾患Note=A chromosomal aberration involving SSX1 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency).
  • 配列類似性Belongs to the SSX family.
    Contains 1 KRAB-related domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cancer/testis antigen 5.1 antibody
    • Cancer/testis antigen family 5 member 1 antibody
    • CT5.1 antibody
    • MGC150425 antibody
    • MGC5162 antibody
    • Protein SSX1 antibody
    • sarcoma, synovial, X chromosome related 1 antibody
    • SSRC antibody
    • SSX1 antibody
    • SSX1_HUMAN antibody
    • Synovial sarcoma antibody
    • Synovial sarcoma, X breakpoint 1 antibody
    • X breakpoint 1 antibody
    see all

Anti-SSX1 antibody [1E10] 画像

  • All lanes : Anti-SSX1 antibody [1E10] (ab119036) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SSX1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 22 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201600)
  • ab119036, at 1/100 dilution, staining SSX1 in COS7 cells transiently transfected with pCMV6-ENTRY SSX1 by Immunofluorescence.
  • HEK293T cells transfected with either a SSX1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab119036 at 1/100 dilution and then analyzed by flow cytometry.
  • Flow cytometric analysis of SSX1 in Hela cells, using ab119036 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).
  • Flow cytometric analysis of SSX1 in Jurkat cells using ab119036 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).

Anti-SSX1 antibody [1E10] (ab119036) 使用論文

ab119036 has not yet been referenced specifically in any publications.

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