製品の概要

  • 製品名Anti-SSH3BP1 antibody
    SSH3BP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SSH3BP1
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residue 325 and 375 of human SSH3BP1 (NP_005461.2).

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa and 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 55, 60 kDa (predicted molecular weight: 55 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level.
  • 組織特異性Widely expressed, with highest expression in brain.
  • 関連疾患Note=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript.
  • 配列類似性Belongs to the ABI family.
    Contains 1 SH3 domain.
    Contains 1 t-SNARE coiled-coil homology domain.
  • ドメインThe t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A.
  • 翻訳後修飾In vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl.
  • 細胞内局在Cytoplasm. Nucleus. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Abelson interactor 1 antibody
    • Abi-1 antibody
    • Abi1 antibody
    • ABI1_HUMAN antibody
    • Abl binding protein 4 antibody
    • Abl interactor 1 antibody
    • Abl interactor protein 1 antibody
    • Abl-binding protein 4 antibody
    • AblBP4 antibody
    • e3B1 antibody
    • Eps8 binding protein antibody
    • Eps8 SH3 domain binding protein antibody
    • Eps8 SH3 domain-binding protein antibody
    • Eps8-binding protein antibody
    • Hssh3bp1 antibody
    • Interactor protein AblBP4 antibody
    • NAP1 antibody
    • Nap1 binding protein antibody
    • Nap1-binding protein antibody
    • Nap1BP antibody
    • Spectrin SH3 domain binding protein 1 antibody
    • Spectrin SH3 domain-binding protein 1 antibody
    • SSH3BP antibody
    see all

Anti-SSH3BP1 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SSH3BP1 with ab72318 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SSH3BP1 antibody (ab72318) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 55 kDa
    Observed band size : 55,60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human SSH3BP1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Lane 1: ab72317 used for IP at 3 µg/mg lysate. Lane 2: SSH3BP1 immunoprecipitated with an other antibody. Lane 3: ab72318 at 3 µg/mg lysate used for IP of SSH3BP1, which recognize downstream epitopes. Lane 4: control Ig IP For blotting immunoprecipitated SSH3BP1, ab72318 was used at 1 µg/ml.
  • Detection of SSH3BP1 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72318 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

Anti-SSH3BP1 antibody (ab72318) 使用論文

ab72318 has not yet been referenced specifically in any publications.

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