Anti-SSEA4 抗体 [MC813-70] (ab16287)
Key features and details
- Mouse monoclonal [MC813-70] to SSEA4
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG3
製品の概要
-
製品名
Anti-SSEA4 antibody [MC813-70] -
製品の詳細
Mouse monoclonal [MC813-70] to SSEA4 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Tissue, cells or virus corresponding to Human SSEA4. Human embryonal carcinoma cell line 2102Ep
-
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide -
Concentration information loading...
-
精製度
Tissue culture supernatant -
特記事項(精製)
Tissue culture supernatant was cross flow concentrated. -
ポリ/モノ
モノクローナル -
クローン名
MC813-70 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG3 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16287の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (8) |
Use at an assay dependent concentration.
|
Flow Cyt | (2) |
Use at an assay dependent concentration.
ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
特記事項 |
---|
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
関連性
Glycosphingolipids function as mediators of cell adhesion and are modulators of signal transduction. SSEA-4 (Stage-Specific Embryonic Antigen 4) is a glycolipid expressed early in embryonic development and in pluripotent stem cells. This antibody was first described and named as part of a series of embryonic antigens, defined by monoclonal antibodies isolated in the lab of Prof. Davor Solter (Kannagi, R. et al., 1983, EMBO J. 2:2355). SSEA-4 can be used as a marker of human embryonic stem cells, human embryonic carcinoma cells and human embryonic germ cells. Monoclonal antibodies to this target have been widely used in the characterization of pluripotent stem cells. Mouse pluripotent stem cells are not recognised by anti-SSEA-4 antibodies but do express the antigen upon differentiation. -
細胞内局在
Cell Membrane -
別名
- Stage specific embryonic antigen 4 antibody
画像
-
Human induced pluripotent stem cells (iPSCs) stained with ab16287 (red fill) with secondary only control (blue fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16287, 1/9600 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG at 1/1000 dilution for 30 min at 4°C. iPSCs differentiated for 14 days toward a cardiomyocyte lineage were used as a negative control. Acquisition of >20,000 total events were collected using a 100mW solid state diode laser (488nm) and 529/28 bandpass filter.
-
ab16287 staining SSEA4 in rat tendon derived stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 20 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
-
Immunofluorescence analysis of Human dental pulp stem cells, staining SSEA4 with ab16287 at 1/40 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.
-
Immunocytochemistry/ Immunofluorescence analysis of human iPSC cells labeling SSEA4 with ab16287 at 1/500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. Cells were blocked with 5% serum for 20 minutes at 25°C. A goat polyclonal anti-mouse Cy3 secondary antibody at 1/500 dilution was used.
-
anti-SSEA4 antibody, ab16297 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. As can be seen from the histograms, in non-differentiating conditions (i.e. without retinoic acid) NTERA2 cells were recognised by ab16297. However, upon differentiation (addition of retinoic acid), the antibody lost the ability to recognise the cells.
-
2102ep Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.
-
ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocking with 10% serum for 1 hour was performed. Samples were incubated with primary antibody (1/100: in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor® 588-conjugated goat polyclonal to mouse IgG was used at dilution at 1/100 as secondary antibody.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (165)
ab16287 は 165 報の論文で使用されています。
- Rabino M et al. Generation of four human induced pluripotent stem cell lines from COVID-19 hospitalized patients with increased levels of cardiac Troponin in the acute infection phase developing or not myocarditis. Stem Cell Res 67:103018 (2023). PubMed: 36630840
- Rodríguez-Polo I et al. Generation and Cultivation of Transgene-Free Macaque and Baboon iPSCs Under Chemically Defined Conditions. Methods Mol Biol 2454:697-716 (2022). PubMed: 33772458
- Linda K et al. Imbalanced autophagy causes synaptic deficits in a human model for neurodevelopmental disorders. Autophagy 18:423-442 (2022). PubMed: 34286667
- Gunaratne GS et al. Identification of a dihydropyridine scaffold that blocks ryanodine receptors. iScience 25:103706 (2022). PubMed: 35059610
- Deschuyter M et al. ST3GAL2 knock-down decreases tumoral character of colorectal cancer cells in vitro and in vivo. Am J Cancer Res 12:280-302 (2022). PubMed: 35141018