製品の概要

  • 製品名Anti-SSEA3 antibody [MC631]
    SSEA3 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [MC631] to SSEA3
  • アプリケーション適用あり: IHC-FoFr, WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    4-8 cell stage mouse embryos.

  • ポジティブ・コントロール
    • 2102Ep Human Embryonic Carcinoma Cells
  • 特記事項The mouse myeloma was fused with a spleen from a Fisher rat.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Contains 0.4M Arginine
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
  • ポリ/モノモノクローナル
  • クローン名MC631
  • ミエローマP3x63-Ag8.653
  • アイソタイプIgM
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/20.

ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.



ab35768-Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性SSEA3 is a globoseries carbohydrate antigen present on both cell surface glycolipids and glycopeptides. It is found on the surface of mouse oocytes, becoming restricted to the intracellular mass of the early blastocyst and then to the primitive endoderm. It is present on the surface of human teratocarcinoma cells (EC), embryonic germ cells (EG) and embryonic stem cells (ES) but not mouse undifferentiated mouse cells of these types. SSEA3 expression decreases as human EC, ES and EG cells differentiate but increases on differentiation of mouse EC, ES and EG cells. It is also found on the surface of mouse erythrocytes.
  • 別名
    • Stage specific embryonic antigen 3 antibody

Anti-SSEA3 antibody [MC631] 画像

  • The image shows cell membrane staining of 2102Ep human embryonic carcinoma cells using SSEA3 specific antibody ab16286.
  • Anti-SSEA3 antibody, ab16286 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. The top histogram (A) shows undifferentiated NTERA2 cells (i.e. without retinoic acid) recognised by ab16286. However, upon differentiation (by the addition of retinoic acid 10-5M for 7 days), the antibody lost the ability to recognise these cells as shown in the bottom histogram (B).

Anti-SSEA3 antibody [MC631] (ab16286) 使用論文

This product has been referenced in:
  • Eid W & Abdel-Rehim W Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A. Biol Chem N/A:N/A (2016). Read more (PubMed: 27343473) »
  • Altman E  et al. Characterization of human spermatogonial stem cell markers in fetal, pediatric, and adult testicular tissues. Reproduction 148:417-27 (2014). Read more (PubMed: 25030892) »

See all 17 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human ES cell (hues7))
Specification human ES cell (hues7)
Fixative Paraformaldehyde
Permeabilization No
Blocking step 1% serum 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
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Abcam user community

Verified customer

投稿 Jun 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (human ES cell undifferentiated)
Specification human ES cell undifferentiated
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: FACS buffer (0.2% BSA, 0.1% sodium azide in PBS)
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy FSC vs SSC on negative control sample
Username

Abcam user community

Verified customer

投稿 Jun 08 2012

Thanks for your reply. I would be happy to send a vial of ab32118. Could you just confirm your shipping address? Thank you, and I look forward to hearing from you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"