The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 106 cells. ab35773-Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.
関連性SSEA3 is a globoseries carbohydrate antigen present on both cell surface glycolipids and glycopeptides. It is found on the surface of mouse oocytes, becoming restricted to the intracellular mass of the early blastocyst and then to the primitive endoderm. It is present on the surface of human teratocarcinoma cells (EC), embryonic germ cells (EG) and embryonic stem cells (ES) but not mouse undifferentiated mouse cells of these types. SSEA3 expression decreases as human EC, ES and EG cells differentiate but increases on differentiation of mouse EC, ES and EG cells. It is also found on the surface of mouse erythrocytes.
Image shows cell surface flow cytometric analysis of SSEA3 on NCCIT cells using 0.5 ug of ab94857. Green histogram represents an isotype control; red represents ab94857. A cell surface flow kit and a PE-conjugated streptavidin secondary were used for this test (cells were not fixed for testing).