製品の概要

  • 製品名Anti-SSB (phospho S366) antibody
    SSB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SSB (phospho S366)
  • 特異性Detects SSB only when phosphorylated at serine 366.
  • アプリケーション適用あり: ELISA, ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphopeptide derived from human SSB around the phosphorylation site of serine 366 (F-A-SP-D-D).

  • ポジティブ・コントロール
    • HeLa cells, extracts from 293 cells This antibody gave a positive result in IHC in the following FFPE tissue: Human breast adenocarcinoma.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

  • 機能Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.
  • 配列類似性Contains 1 HTH La-type RNA-binding domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Phosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
    The N-terminus is blocked.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Autoantigen La antibody
    • La antibody
    • La autoantigen antibody
    • La autoantigen homolog antibody
    • La protein antibody
    • La ribonucleoprotein antibody
    • La ribonucleoprotein domain family member 3 antibody
    • LA_HUMAN antibody
    • LARP3 antibody
    • Lupus La antigen antibody
    • Lupus La protein antibody
    • Lupus La protein homolog antibody
    • MGC118101 antibody
    • MGC93380 antibody
    • mRNA for autoantigen antibody
    • OTTMUSP00000014043 antibody
    • RP23 273G23.1 antibody
    • Sjoegren syndrome type B antigen antibody
    • Sjogren syndrome antigen B (autoantigen La) antibody
    • Sjogren syndrome antigen B antibody
    • SS B antibody
    • SS-B antibody
    • SS-B/La protein antibody
    • SSB antibody
    see all

Anti-SSB (phospho S366) antibody 画像

  • IHC image of SSB (phospho S366) staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61800, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-SSB (phospho S366) antibody (ab61800) at 1/500 dilution

    Lane 1 : extracts from 293 cells
    Lane 2 : extracts from 293 cells with immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • Ab61800 at 1/500 dilution staining HeLa cells, (left) treated with Forskolin (40nM, 30mins), (right) untreated cells.

Anti-SSB (phospho S366) antibody (ab61800) 使用論文

ab61800 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"