製品の概要

  • 製品名Anti-SRPR beta antibody
    SRPR beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SRPR beta
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 181-230 (QDIAMAKSAK LIQQQLEKEL NTLRVTRSAA PSTLYSSSTA PAQLGKKGKE) of Human SRPR beta (NP_067026).

  • ポジティブ・コントロール
    • Hela cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab81324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.

IHC-P 1/100.

ターゲット情報

  • 機能Component of the SRP (signal recognition particle) receptor. Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system. Has GTPase activity. May mediate the membrane association of SRPR.
  • 配列類似性Belongs to the SRP receptor beta subunit family.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APMCF1 antibody
    • Protein APMCF1 antibody
    • Signal recognition particle receptor B subunit antibody
    • Signal recognition particle receptor beta subunit antibody
    • Signal recognition particle receptor subunit beta antibody
    • SR-beta antibody
    • SRbeta antibody
    • SRPR beta antibody
    • Srprb antibody
    • SRPRB_HUMAN antibody
    see all

Anti-SRPR beta antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling SRPR beta with ab81324 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SRPR beta. Right - Merge.


  • Predicted band size : 30 kDa
    Western blot analysis of Jurkat cell lysates labeling SRPR beta with ab81324 at 1.0µg/ml


  • Predicted band size : 30 kDa
    Western blot analysis of 721_B cell lysates labeling SRPR beta with ab81324 at 1.0µg/ml
  • Anti-SRPR beta antibody (ab81324) at 1 µg/ml + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

Anti-SRPR beta antibody (ab81324) 使用論文

ab81324 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"