製品の概要

  • 製品名Anti-SRPK2 antibody
    SRPK2 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to SRPK2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length SRPK2 protein (Human)

  • ポジティブ・コントロール
    • SRPK2 transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 75, 140 kDa (predicted molecular weight: 78 kDa).

ターゲット情報

  • 機能Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.
  • 組織特異性Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylation at Thr-492 by PKB/AKT1 enhances its stimulatory activity in triggering cyclin-D1 (CCND1) expression and promoting apoptosis in neurons, which can be blocked by YWHAB. It also enhances its protein kinase activity toward ACIN1 and SRSF2, promotes its nuclear translocation and prevents its proteolytic cleavage.
    Proteolytically cleaved at Asp-139 and Asp-403 by caspase-3 during apoptotic cell death. Cleavage at Asp-139 which is the major site of cleavage, produces a small N-terminal fragment that translocates into nucleus and promotes VP16-induced apoptosis.
  • 細胞内局在Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. KAT5/TIP60 inhibits its nuclear translocation. Phosphorylation at Thr-492 by PKB/AKT1 promotes nuclear translocation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Human serine kinase SRPK2 mRNA, complete cds antibody
    • Serine kinase SRPK2 antibody
    • Serine/arginine rich protein specific kinase 2 antibody
    • Serine/arginine-rich protein-specific kinase 2 antibody
    • Serine/threonine protein kinase SRPK2 antibody
    • SFRS protein kinase 2 antibody
    • SFRSK2 antibody
    • SR protein specific kinase 2 antibody
    • SR-protein-specific kinase 2 antibody
    • SRPK2 antibody
    • SRPK2_HUMAN antibody
    • SRSF protein kinase 2 C-terminal antibody
    see all

Anti-SRPK2 antibody 画像

  • All lanes : Anti-SRPK2 antibody (ab67993) at 1/500 dilution

    Lane 1 : SRPK2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 78 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.

Anti-SRPK2 antibody (ab67993) 使用論文

This product has been referenced in:
  • Huang Y  et al. Global tumor protein p53/p63 interactome: Making a case for cisplatin chemoresistance. Cell Cycle 11:2367-79 (2012). WB ; Human . Read more (PubMed: 22672905) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab67993.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"