Anti-SRPK1 抗体 (ab90527)
Key features and details
- Rabbit polyclonal to SRPK1
- Suitable for: ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SRPK1 antibody
SRPK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SRPK1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide within Human SRPK1 aa 275-325 (internal sequence). The exact sequence is proprietary. (GeneID 6732)
Database link: NP_003128.3 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab90527の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 92 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 92 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. Locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. Appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids. -
組織特異性
Isoform 2 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 is only seen in the testis, at lower levels than isoform 2. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6732 Human
- Entrez Gene: 20815 Mouse
- Entrez Gene: 361811 Rat
- Omim: 601939 Human
- SwissProt: Q96SB4 Human
- SwissProt: O70551 Mouse
- Unigene: 443861 Human
- Unigene: 15252 Mouse
see all -
別名
- Serine/arginine rich protein specific kinase 1 antibody
- Serine/arginine rich splicing factor kinase 1 antibody
- Serine/arginine-rich protein-specific kinase 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling SRPK1 with ab90527 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-SRPK1 antibody (ab90527) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 92 kDa
Exposure time: 10 seconds -
ab90527 at 1 µg/ml detecting SRPK1 following immunoprecipitation from HeLa cell lysate. Detection by ECL with a 3 second exposure.
Lane 1: HeLa cell lysate IP with ab90527
Lane 2: HeLa cell lysate IP with an antibody to a downstream epitope of SRPK1
Lane 3: HeLa cell lysate IP with control IgGAll antibodies used at at 3 µg/mg lysate for immunoprecipitation. 1 mg of lysate was used for IP and 20% of IP loaded.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab90527 は 5 報の論文で使用されています。
- Andrés-Benito P et al. Host Tau Genotype Specifically Designs and Regulates Tau Seeding and Spreading and Host Tau Transformation Following Intrahippocampal Injection of Identical Tau AD Inoculum. Int J Mol Sci 23:N/A (2022). PubMed: 35054902
- Li ZR et al. SOX2 knockdown inhibits the migration and invasion of basal cell carcinoma cells by targeting the SRPK1-mediated PI3K/AKT signaling pathway. Oncol Lett 17:1617-1625 (2019). PubMed: 30675221
- Yan B et al. Long non-coding RNA phosphatase and tensin homolog pseudogene 1 suppresses osteosarcoma cell growth via the phosphoinositide 3-kinase/protein kinase B signaling pathway. Exp Ther Med 15:4829-4837 (2018). PubMed: 29805503
- Tzelepis K et al. SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4. Nat Commun 9:5378 (2018). PubMed: 30568163
- Huang Y et al. Global tumor protein p53/p63 interactome: Making a case for cisplatin chemoresistance. Cell Cycle 11:2367-79 (2012). PubMed: 22672905