製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to SRPK1
  • アプリケーション
    適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: NETLRHKEDL HNANDCDVQN LNQESSFLSS QNGDSSTSQE TDSCTPITSE VSDTMVCQSS STVGQSFSEQ HISQLQESIR AEIPCEDEQE QEHNGPLDNK , corresponding to amino acids 371-470 of Human SRPK1

  • ポジティブ・コントロール
    • This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: HeLa (meth),HepG2 (meth), MCF-7 (meth), HepG2 (PFA).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.01µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 92 kDa.Can be blocked with Recombinant Human SRPK1 protein (ab152706).
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. Locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. Appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.
  • 組織特異性
    Isoform 2 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 is only seen in the testis, at lower levels than isoform 2.
  • 配列類似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Serine/arginine rich protein specific kinase 1 antibody
    • Serine/arginine rich splicing factor kinase 1 antibody
    • Serine/arginine-rich protein-specific kinase 1 antibody
    • Serine/threonine protein kinase SRPK1 antibody
    • Serine/threonine-protein kinase SRPK1 antibody
    • SFRS protein kinase 1 antibody
    • SFRSK1 antibody
    • SR protein kinase 1 antibody
    • SR protein specific kinase 1 antibody
    • SR-protein-specific kinase 1 antibody
    • SRPK1 antibody
    • SRPK1_HUMAN antibody
    see all

Anti-SRPK1 antibody 画像

  • Overlay histogram showing PANC1 cells stained with ab58002 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58002, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ICC/IF image of ab58002 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58002 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) HepG2 and HeLa cells at 5ug/ml, also in formaldehyde fixed (4%, 10min) HepG2 cells at 5ug/ml.

  • SRPK1 antibody (ab58002) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.


  • Predicted band size : 92 kDa
    SRPK1 antibody (ab58002) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.

Anti-SRPK1 antibody (ab58002) 使用論文

ab58002 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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