Anti-SRGAP1 抗体 (ab76926)
Key features and details
- Rabbit polyclonal to SRGAP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SRGAP1 antibody
SRGAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SRGAP1 -
由来種
Rabbit -
特異性
Does not crossreact with human srGAP2 or human srGAP3 -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat, Elephant -
免疫原
Synthetic peptide corresponding to a region between residue 979 and 1029 of human SRGAP1 (NP_065813.1)
-
ポジティブ・コントロール
- HeLa and 293T whole cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab76926 was affinity purified using an epitope specific to SRGAP1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76926の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
|
IP |
Use at 2-5 µg/mg of lysate.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
機能
GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42. -
組織特異性
Expressed in brain, lung, kidney, and testis. -
配列類似性
Contains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain. -
ドメイン
The F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions. - Information by UniProt
-
参照データベース
- Entrez Gene: 57522 Human
- Omim: 606523 Human
- SwissProt: Q7Z6B7 Human
- Unigene: 210751 Human
- Unigene: 593803 Human
-
別名
- 4930572H05Rik antibody
- ARHGAP13 antibody
- FLJ22166 antibody
see all
画像
-
All lanes : Anti-SRGAP1 antibody (ab76926) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate. at 50 µg
Lane 2 : HeLa whole cell lysate. at 15 µg
Lane 3 : HeLa whole cell lysate. at 5 µg
Lane 4 : 293T whole cell lysate. at 50 µg
Predicted band size: 124 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands. -
ab76926 at 1µg/ml staining SRGAP1 in HeLa whole cell lysate immunoprecipitated using ab76926 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab76926 は 2 報の論文で使用されています。
- Chiang TS et al. ADP-ribosylation factor-like 4A interacts with Robo1 to promote cell migration by regulating Cdc42 activation. Mol Biol Cell 30:69-81 (2019). PubMed: 30427759
- Genet G et al. Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis. Nat Commun 10:2350 (2019). PubMed: 31138815