製品の概要

  • 製品名Anti-SREBP2 antibody
    SREBP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SREBP2
  • アプリケーション適用あり: IHC-Fr, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
    交差が予測される動物種: Guinea pig, Pig, Xenopus laevis, Chinese Hamster
  • 免疫原

    Synthetic peptide corresponding to Human SREBP2 aa 455-469.
    Sequence:

    SPLLDDAKVKDEPDS


    Database link: Q12772
    (Peptide available as ab174740)

  • ポジティブ・コントロール
    • Rat testis supernatant.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/100 - 1/150.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/200 - 1/1000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).Can be blocked with SREBP2 peptide (ab174740).

Block with 5% non-fat dry milk in TBS pH 7.4 for 1-2 hours at room temperature or over night at 4°C.

ターゲット情報

  • 機能Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
  • 組織特異性Ubiquitously expressed in adult and fetal tissues.
  • 配列類似性Belongs to the SREBP family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻訳後修飾At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
  • 細胞内局在Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI608257 antibody
    • bHLHd2 antibody
    • Class D basic helix-loop-helix protein 2 antibody
    • OTTHUMP00000028740 antibody
    • Processed sterol regulatory element-binding protein 2 antibody
    • SRBP2_HUMAN antibody
    • SREBF 2 antibody
    • Srebf2 antibody
    • SREBF2 protein antibody
    • SREBP 2 antibody
    • SREBP-2 antibody
    • SREBP2 antibody
    • SREBP2gc antibody
    • sterol regulatory element binding factor 2 antibody
    • Sterol regulatory element binding protein 2 antibody
    • Sterol regulatory element binding transcription factor 2 antibody
    • Sterol regulatory element-binding transcription factor 2 antibody
    see all

Anti-SREBP2 antibody 画像

  • Anti-SREBP2 antibody (ab30682) at 1/1000 dilution + Human retina whole cell lysate at 30 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 126 kDa
    Additional bands at : 60 kDa (possible non-specific binding).

    Exposure time : 6 minutes

    This image is courtesy of an Abreview by Dongil Kim.

    Blocking: 7% milk for 60 minutes at 20oC.

  • All lanes : Anti-SREBP2 antibody (ab30682) at 1/200 dilution

    Lane 1 : Human fibroblast cell lysate
    Lane 2 : Rat brown fat homogenate
    Lane 3 : Rat testis supernatant

    Lysates/proteins at 60 µg per lane.


    Predicted band size : 126 kDa
    Observed bands 126 kDa, 55kDa (cleaved form)
  • Immunohistochemistry (Frozen sections) analysis of wild type and ISR2 transgenic mouse jejunum tissue sections labelling SREBP2 with ab30682 at a dilution of 1/100. Sections (5–10 µm thickness) were cut from snap-frozen tissues embedded in OCT medium using a cryostat and were mounted on the slides and preserved at -80°C. Sections were fixed with 4% paraformaldehyde in PBS for 20 min at room temperature followed by blocking in PBS containing 5% normal goat serum at room temperature. Sections were then incubated with the primary antibody in the blocking buffer. After PBS washes, the sections were incubated with the secondary antibody, an Alexa Fluor® 568-conjugated goat anti-rabbit IgG (red) for 60 min and then washed and mounted with slow-fade DAPI (blue, nuclei) by using coverslips. Microscopy was performed with a 20× oil immersion objective of Zeiss immunofluorescence microscope (Observer Z1) equipped with deconvolution software (AxioVision).

    The figure shows predominant cytoplasmic staining in wild type mice and increased colocalization of SREBP2 with the nuclei in ISR2 mice (white arrow).

  • ICC/IF image of ab30682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SREBP2 antibody (ab30682) 使用論文

This product has been referenced in:
  • Mustafa M  et al. SREBP inhibition ameliorates renal injury after unilateral ureteral obstruction. Am J Physiol Renal Physiol 311:F614-25 (2016). Read more (PubMed: 27385736) »
  • Zheng W  et al. Pathways of cholesterol homeostasis in mouse retina responsive to dietary and pharmacologic treatments. J Lipid Res 56:81-97 (2015). Read more (PubMed: 25293590) »

See all 26 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Specification Liver
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 May 18 2016

Application Western blot
Sample Human Cell lysate - whole cell (SCC61)
Gel Running Conditions Reduced Denaturing
Loading amount 48 µg
Treatment As indicated on image
Specification SCC61
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 28 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Suypriya Sen

Verified customer

投稿 May 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (retina)
Gel Running Conditions Reduced Denaturing (8%)
Loading amount 30 µg
Specification retina
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

投稿 Apr 11 2013

Ich möchte vorschlagen, dass Sie die Kreuzreaktion zu vermerken, damit anderen Kunden wissen, dass mit Merschweinchenproben mit Problemen zu rechnen ist.

Vielen Dank für Ihre Anfrage.
Die Interpretation Ihrer Ergebnisse ist wirklich nicht einfach! Ich hoffe, dass ich Ihnen trotzdem ein wenig weiterhelfen kann:
Zuerst möchte ich fragen, welche unserer SREBP- Antikörper Sie verwendet haben. Aus ...

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Code de remise : ******* Date d’expiration : JJ MM AAAA Je suis ravie que vous souhaitiez participer a notre offre et tester ab30682 chez le hamster chinois. Le code de réduction correspondant est ********. Ce code de réduction est utilisable sous...

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Thank you for your reply. I would recommend testing a fresh aliquot and dilution. I agree that most antibodies can be reused several times and are okay to store at 4C with sodium azide. This may be a case in which these antibodies can only be used...

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Thank you for contacting Abcam regarding ab3259 and ab30682. I want to clarify your initial question regarding your usage of these antibodies. When you say that they worked well for the first 2 incubations - are you reusing the diluted primary anti...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (primary rat hepatocytes)
Loading amount 100 µg
Specification primary rat hepatocytes
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 18 2011

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"