製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SREBP1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the Center region of Human SREBF1 (NP_004167.3).

  • ポジティブ・コントロール
    • MDAMB435 cell line lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 122 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
    • 組織特異性
      Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
    • 配列類似性
      Belongs to the SREBP family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • 翻訳後修飾
      At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
      Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.
    • 細胞内局在
      Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ADD 1 antibody
      • bHLHd1 antibody
      • Class D basic helix-loop-helix protein 1 antibody
      • D630008H06 antibody
      • Processed sterol regulatory element-binding protein 1 antibody
      • SRBP1_HUMAN antibody
      • SREBF 1 antibody
      • SREBF1 antibody
      • SREBP 1 antibody
      • SREBP 1c antibody
      • SREBP-1 antibody
      • SREBP1 antibody
      • Sterol regulatory element binding protein 1 antibody
      • Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 antibody
      • Sterol regulatory element binding transcription factor 1 antibody
      • Sterol regulatory element-binding transcription factor 1 antibody
      see all

    画像

    • Anti-SREBP1 antibody (ab93333) at 1/100 dilution + MDAMB435 cell line lysates at 35 µg

      Predicted band size : 122 kDa

    プロトコール

    参考文献

    ab93333 has not yet been referenced specifically in any publications.

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