製品の概要

  • 製品名Anti-SREBP1 antibody [2A4]
    SREBP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2A4] to SREBP1
  • アプリケーション適用あり: Flow Cyt, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Guinea pig, Cow, Human
  • 免疫原

    Recombinant fragment corresponding to Human SREBP1 aa 301-407 (N terminal). Recombinant fragment, corresponding to N terminal amino acids 301/407 (the bHLH/leucine zipper domain) of human SREBP-1.
    Database link: P36956

  • エピトープAmino acids 301 - 407.
  • ポジティブ・コントロール
    • IMR 5 and HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 125 kDa (predicted molecular weight: 127 kDa).

Detects a band of approximately 125 kDa (predicted molecular weight: 127 kDa). By Western blot, this antibody detects a band of 125 kDa (precursor), which corresponds to the predicted molecular weight of SREBP 1 and 60 - 70 kDa(cleaved).

ターゲット情報

  • 機能Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
  • 組織特異性Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
  • 配列類似性Belongs to the SREBP family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻訳後修飾At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
    Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.
  • 細胞内局在Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADD 1 antibody
    • bHLHd1 antibody
    • Class D basic helix-loop-helix protein 1 antibody
    • D630008H06 antibody
    • Processed sterol regulatory element-binding protein 1 antibody
    • SRBP1_HUMAN antibody
    • SREBF 1 antibody
    • SREBF1 antibody
    • SREBP 1 antibody
    • SREBP 1c antibody
    • SREBP-1 antibody
    • SREBP1 antibody
    • Sterol regulatory element binding protein 1 antibody
    • Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 antibody
    • Sterol regulatory element binding transcription factor 1 antibody
    • Sterol regulatory element-binding transcription factor 1 antibody
    see all

Anti-SREBP1 antibody [2A4] 画像

  • Anti-SREBP1 antibody [2A4] (ab3259) at 1/1000 dilution + whole cell lysate prepared from murine macrophages at 30 µg

    Secondary
    HRP conjugated goat anti-mouse IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 127 kDa


    Exposure time : 3 minutes

    Image courtesy of an anonymous Abreview.

    The two bands have the expected size corresponding to the full and cleaved forms of SREBP1.

    See Abreview

  • ICC/IF image of ab3259 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing THP1 cells stained with ab3259 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3259, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ICC/IF image of ab3259 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SREBP1 antibody [2A4] (ab3259) 使用論文

This product has been referenced in:
  • Carrier B  et al. Alpha-lipoic acid reduces LDL-particle number and PCSK9 concentrations in high-fat fed obese Zucker rats. PLoS One 9:e90863 (2014). WB ; Rat . Read more (PubMed: 24595397) »
  • Rauer C  et al. Sterol regulatory element-binding proteins are regulators of the rat thyroid peroxidase gene in thyroid cells. PLoS One 9:e91265 (2014). WB ; Human . Read more (PubMed: 24625548) »

See all 12 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (LIVER)
Gel Running Conditions Reduced Denaturing (5)
Loading amount 50 µg
Specification LIVER
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 17 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (HRMEC)
Specification HRMEC
Treatment OxLDL for 15h
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Feb 16 2015

Application ChIP
Detection step Real-time PCR
Sample Mouse Tissue lysate - other (Liver)
Specification Liver
Negative control Control IgG
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 40 minute(s) and 0 second(s)
Specification of the cross-linking agent: 40 minutes DSG + 10 minutes pa
Positive control Specific de novo lipogenesis inducing Chow that demonstrated an increase in SREBP1c protein expression in nuclear extract and an increase in Fasn (SREBP1c target) gene expression.
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投稿 Nov 05 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (gel 8%)
Sample Cow Cell lysate - whole cell (Bovine mammary epithelial cells)
Specification Bovine mammary epithelial cells
Treatment 150nM tran10,cis12 CLA for 24hrs
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Miss. xinbei huang

Verified customer

投稿 Jul 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (liver)
Loading amount 30 µg
Specification liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: rt°C
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Verified customer

投稿 Jan 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1 monocyte-derived macrophages)
Loading amount 50 µg
Specification THP-1 monocyte-derived macrophages
Treatment TO-901317
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Elizabeth Kilby

Verified customer

投稿 Jan 04 2013

Muchas gracias por contactarnos de nuevo.

He llevado a cabo un BLAST entre las secuencias del inmunógeno usado para generar ab3259 y la secuencia de la proteína en conejo, y el porcentaje de homología es bastante elevado ...

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Thank you for contacting us. I am sorry that ab3259 has been detecting bands at incorrect molecular weights. While I would typically recommend loading between 20-40 ug of whole cell lysate or 10-15 ug of nuclear lysate per lane to ensure the maximum sp...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital informa...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Rat liver tissue)
Loading amount 140 µg
Specification Rat liver tissue
Gel Running Conditions Reduced Denaturing (7%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Smitha Koshy

Verified customer

投稿 Jul 23 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"