製品の概要

  • 製品名Anti-Src (phospho Y529) antibody
    Src 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Src (phospho Y529)
  • 特異性Fyn and Yes (92%homologous) were not tested.
  • アプリケーション適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide (Human) derived from the region of Src that contains tyrosine 529, based on the human sequence. The sequence is conserved in mouse (tyrosine 534), chicken (tyrosine 527) and frog (tyrosine 525).

  • ポジティブ・コントロール
    • Chicken Embryo Fibroblast (CEF) cells expressing Src protein and plated on fibronectin.
  • 特記事項


    Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of the catalytic domain residue (tyrosine 418 in the human sequence), thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylatedleading to full activation.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Src, and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a Src-derived peptide that is phosphorylated at tyrosine 529.
  • 一次抗体 備考Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of the catalytic domain residue (tyrosine 418 in the human sequence), thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylatedleading to full activation.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

ターゲット情報

  • 機能Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.
  • 細胞内局在Cell membrane. Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 別名
    • ASV antibody
    • Avian sarcoma virus antibody
    • c SRC antibody
    • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
    • cSrc antibody
    • EC 2.7.10.2 antibody
    • Neuronal CSRC tyrosine specific protein kinase antibody
    • Neuronal SRC antibody
    • Oncogene SRC antibody
    • OTTHUMP00000174476 antibody
    • OTTHUMP00000174477 antibody
    • p60 Src antibody
    • p60-Src antibody
    • p60Src antibody
    • pp60c src antibody
    • pp60c-src antibody
    • pp60csrc antibody
    • Proto oncogene tyrosine protein kinase Src antibody
    • Proto-oncogene c-Src antibody
    • Proto-oncogene tyrosine-protein kinase Src antibody
    • Protooncogene SRC antibody
    • Protooncogene SRC Rous sarcoma antibody
    • Src antibody
    • SRC Oncogene antibody
    • SRC proto oncogene non receptor tyrosine kinase antibody
    • SRC_HUMAN antibody
    • SRC1 antibody
    • Tyrosine kinase pp60c src antibody
    • Tyrosine protein kinase SRC 1 antibody
    • Tyrosine protein kinase SRC1 antibody
    • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
    • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
    • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
    see all

Anti-Src (phospho Y529) antibody 画像



  • Predicted band size : 62 kDa

    Dr. Michael Schaller, Dept. of Cell Bio. & Anat., Univ. of North Carolina, Chapel Hill, NC.

    Western blot detection of Src phosphorylation in extracts of chick embryo fibroblasts expressing wild-type (lanes 2,4,6) or mutant (lanes 1,3,5) pp60src. Truncation of Src at position 518 eliminated phosphorylation at the negative regulatory site [pY529], while increasing phosphorylation at the catalytic site [pY418].

     

     

     

    Western blot detection of Src phosphorylation in extracts of chick embryo fibroblasts expressing wild-type (lanes 2,4,6) or mutant (lanes 1,3,5) pp60src. Truncation of Src at position 518 eliminated phosphorylation at the negative regulatory site [pY529], while increasing phosphorylation at the catalytic site [pY418].

Anti-Src (phospho Y529) antibody (ab4817) 使用論文

This product has been referenced in:
  • Xiao X  et al. c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution. Am J Physiol Endocrinol Metab 304:E145-59 (2013). WB ; Rat . Read more (PubMed: 23169788) »

See 1 Publication for this product

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For ab4817, ab32078 the immuinogen peptide are unfortunately not available.

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Thank you very much for your patience. The batches of ab4816 and ab4817 that we currently have in stock are at a concentration of 0.25 mg/mL. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"