製品の概要

  • 製品名Anti-Src (phospho Y215) antibody
    Src 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Src (phospho Y215)
  • 特異性ab24789 recognises c-Src and it may recognise other Src family members such as hck, fyn, csk and yes when phosphorylated on their conserved SH-2 domain tyrosine site.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human) corresponding to amino acids around tyrosine 215 of human Src ,conjugated to KLH

  • ポジティブ・コントロール
    • A431 cells treated with pervanadate
  • 特記事項


    This antibody is useful in the study of tyrosine kinase regulation to detect phosphorylated Src. Phosphorylation of Tyr-215 (and Tyr-418) increase tyrosine kinase activity.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 60 kDa.

Incubate the blotting membrane with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.

ELISA 1/2000.

ターゲット情報

  • 機能Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.
  • 細胞内局在Cell membrane. Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 別名
    • ASV antibody
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    • Proto oncogene tyrosine protein kinase Src antibody
    • Proto-oncogene c-Src antibody
    • Proto-oncogene tyrosine-protein kinase Src antibody
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    • Src antibody
    • SRC Oncogene antibody
    • SRC proto oncogene non receptor tyrosine kinase antibody
    • SRC_HUMAN antibody
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    • Tyrosine kinase pp60c src antibody
    • Tyrosine protein kinase SRC 1 antibody
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    • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
    • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
    • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
    see all

Anti-Src (phospho Y215) antibody 画像

  • All lanes : Anti-Src (phospho Y215) antibody (ab24789) at 1/1000 dilution

    Lane 1 : A431 cells treated with pervanadate (1 mM) for 30 minutes (20 ug/lane) with absence of blocking peptide
    Lane 2 : A431 cells treated with pervanadate (1 mM) for 30 minutes (20 ug/lane) with presence of phospho-c-Src (Tyr-215) peptide
    Lane 3 : A431 cells treated with pervanadate (1 mM) for 30 minutes (20 ug/lane) with presence of unrelated phospho-beta-Catenin (Tyr-142) peptide
    Lane 4 : A431 cells treated with pervanadate (1 mM) for 30 minutes (20 ug/lane) with BSA conjugated to phospho-tyrosine


    Predicted band size : 60 kDa

Anti-Src (phospho Y215) antibody (ab24789) 使用論文

This product has been referenced in:
  • Smida M  et al. A novel negative regulatory function of the phosphoprotein associated with glycosphingolipid-enriched microdomains: blocking Ras activation. Blood 110:596-615 (2007). Read more (PubMed: 17389760) »

See 1 Publication for this product

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