製品の概要

  • 製品名Anti-Src antibody
    Src 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Src
  • 特異性This antibody detects endogenous levels of total Src protein. It will react with Src (pY529), Yes (pY538), and Fyn (Y531).
  • アプリケーション適用あり: ICC/IF, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
  • 免疫原

    Synthesized non-phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529.

  • ポジティブ・コントロール
    • Human breast carcinoma tissue; HEK-293 cell extracts

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/10000.

Peptide ELISA only.

IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.
  • 細胞内局在Cell membrane. Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 別名
    • ASV antibody
    • Avian sarcoma virus antibody
    • c SRC antibody
    • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
    • cSrc antibody
    • EC 2.7.10.2 antibody
    • Neuronal CSRC tyrosine specific protein kinase antibody
    • Neuronal SRC antibody
    • Oncogene SRC antibody
    • OTTHUMP00000174476 antibody
    • OTTHUMP00000174477 antibody
    • p60 Src antibody
    • p60-Src antibody
    • p60Src antibody
    • pp60c src antibody
    • pp60c-src antibody
    • pp60csrc antibody
    • Proto oncogene tyrosine protein kinase Src antibody
    • Proto-oncogene c-Src antibody
    • Proto-oncogene tyrosine-protein kinase Src antibody
    • Protooncogene SRC antibody
    • Protooncogene SRC Rous sarcoma antibody
    • Src antibody
    • SRC Oncogene antibody
    • SRC proto oncogene non receptor tyrosine kinase antibody
    • SRC_HUMAN antibody
    • SRC1 antibody
    • Tyrosine kinase pp60c src antibody
    • Tyrosine protein kinase SRC 1 antibody
    • Tyrosine protein kinase SRC1 antibody
    • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
    • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
    • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
    see all

Anti-Src antibody 画像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src antibody, in the presence and absence of blocking peptide.
  • ab47405 staining Src in human HEK-293 cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.5% Triton X-100 and then blocked using 5% serum for 20 minutes at 25°C. Samples were then incubated with primary antibody at 1/1000 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/5000 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

  • ab47405 staining Src in Pig retinal pigment epithelium primary cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in 0.1% TX100, 1% goat serum, 1XPBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/5000) was used as the secondary antibody. Nuclei were counterstained with DAPI.

    See Abreview


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
  • ICC/IF image of ab47405 stained MCF7 cells (ab3871). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47405, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Src antibody (ab47405) at 1/500 dilution

    Lane 1 : HEK-293 cell extracts
    Lane 2 : HEK-293 cell extracts with Blocking peptide


    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Western blot analysis of extracts from 293 cells using Src antibody in the presence and absence of blocking peptide. Western blot analysis of extracts from HEK-293 cells using Src antibody at 1/500 in the presence and absence of blocking peptide.

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa


    Exposure time : 10 seconds
  • Anti-Src antibody (ab47405) at 1/1000 dilution + whole cell lysate prepared from mouse primary astrocytes at 15 µg

    Secondary
    Goat abti-rabbit IgG conjugated to HRP at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa


    Exposure time : 5 seconds

    This image is courtesy of an anonymous abreview.

    Primary antibody incubated for 1 hour at 25°C.
    Blocked with 3& BSA for 1 hour at 25°C.
    Gel run under denaturing conditions.
    Detection method: ImmunoStar.

    See Abreview

Anti-Src antibody (ab47405) 使用論文

This product has been referenced in:
  • Villacis RA  et al. Gene expression profiling in leiomyosarcomas and undifferentiated pleomorphic sarcomas: SRC as a new diagnostic marker. PLoS One 9:e102281 (2014). IHC-P ; Human . Read more (PubMed: 25028927) »
  • Dou X & Charness ME Effect of lipid raft disruption on ethanol inhibition of l1 adhesion. Alcohol Clin Exp Res 38:2707-11 (2014). ICC/IF . Read more (PubMed: 25421507) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Tissue lysate - whole (Kidney)
Specification Kidney
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 05 2013

Thank you for contacting us.

This antibody has been used previously using 10 mM citrate buffer (pH 6.0) for antigen retrieval. This was done in a pressure cooker for 5 minutes. You may need to optimise this for your particular tissue and fix...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat cerebellum primary cells)
Specification Rat cerebellum primary cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TX100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

投稿 Sep 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (Pig retinal pigment epithelium (RPE) primary cells)
Specification Pig retinal pigment epithelium (RPE) primary cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TX100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

投稿 Apr 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK-293 cells)
Specification HEK-293 cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TX100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

投稿 Apr 08 2010

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Loading amount 20 µg
Specification HEK293 cells
Gel Running Conditions Reduced Denaturing (10% SDS)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

投稿 Mar 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse primary astrocytes)
Loading amount 15 µg
Specification mouse primary astrocytes
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"