Application
Immunohistochemistry (Frozen sections)
Blocking step
0,25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample
Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification
mouse embryonic spinal cord E11
Permeabilization
Yes - 0,25%TritonX100
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0,25%TritonX100, 0.2%Gelatine
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: donkey anti rabbit Cy3 Jackson Immunoresearch
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/400
Additional data
Additional Notes
IHC on 20µm frozen section of spinal cord.
Embryos were fixed by immersion in 4% paraformaldehyde in 0.12 M phosphate buffer, pH 7.4 (PFA) and then cryoprotected in 10% sucrose. Cryostat sections were blocked in 0.2% gelatin in PBS containing 0.25% Triton X-100 and incubated overnight at room temperature in the same buffer.
Abcam response
Thank you for your valuable data. It appears that under these experimental conditions, ab121977 did not produce the expected staining pattern. We welcome further customer feedback, please contact Scientific Support for further information.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alain Chedotal
Verified customer
投稿 May 17 2013