製品の概要
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製品名Anti-SQSTM1 / p62 antibody
SQSTM1 / p62 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to SQSTM1 / p62
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由来種Rabbit
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アプリケーション適用あり: WB, IP, IHC-Pmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Sheep, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Immunogen corresponds to a region within amino acids 375-425 of Human SQSTM1/ p62 (NP_003891.1)
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ポジティブ･コントロール
- whole HeLa cells lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab101266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 48 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
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組織特異性Ubiquitously expressed.
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関連疾患Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.
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配列類似性Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger. -
ドメインThe UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.
The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.
The ZZ-type zinc finger mediates the interaction with RIPK1. -
翻訳後修飾Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.
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細胞内局在Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.
- Information by UniProt
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参照データベース
- Entrez Gene: 338045 Cow
- Entrez Gene: 8878 Human
- Entrez Gene: 18412 Mouse
- Entrez Gene: 100172461 Orangutan
- Entrez Gene: 113894 Rat
- Entrez Gene: 705481 Rhesus monkey
- Entrez Gene: 100135450 Sheep
- Omim: 601530 Human
see all -
別名
- A170 antibody
- DMRV antibody
- EBI 3 associated protein of 60 kDa antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab101266)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling SQSTM1 / p62 with ab101266 at 1/1000 (1µg/ml). Detection: Peroxidase Substrate. -
Western blot - Anti-SQSTM1 / p62 antibody (ab101266)All lanes : Anti-SQSTM1 / p62 antibody (ab101266) at 0.1 µg/ml
Lane 1 : whole HeLa cells lysate at 50 µg
Lane 2 : whole HeLa cells lysate at 15 µg
Lane 3 : whole HeLa cells lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-SQSTM1 / p62 antibody (ab101266)Detection of Human SQSTM1 / p62 By Immunoprecipitation in 1mg whole HeLa cells lysate (20% of IP loaded) using ab101266 at 3µg/mg of lysate. Subsequent WB detection was performed using ab101266 at 0.4µg/ml.
参考文献
This product has been referenced in:
- Zhuge X et al. A Novel PhoP/PhoQ Regulation Pathway Modulates the Survival of Extraintestinal Pathogenic Escherichia coli in Macrophages. Front Immunol 9:788 (2018). Read more (PubMed: 29719540) »
- Shen M et al. FSH protects mouse granulosa cells from oxidative damage by repressing mitophagy. Sci Rep 6:38090 (2016). WB ; Mouse . Read more (PubMed: 27901103) »
レビューと Q&A
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