Immunogen corresponds to a region within amino acids 375-425 of Human SQSTM1/ p62 (NP_003891.1)
Our Abpromise guarantee covers the use of ab101266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | 1/2000 - 1/10000. Predicted molecular weight: 48 kDa. | |
IP | Use at 2-5 µg/mg of lysate. | |
IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"