製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to SQRDL
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    SQRDL (NP_067022) full length human protein

  • ポジティブ・コントロール
    • SQRDL transfected 293T cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CACO-2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.

ターゲット情報

  • 機能
    Catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.
  • 配列類似性
    Belongs to the SQRD family.
  • 細胞内局在
    Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 0610039J17Rik antibody
    • 4930557M22Rik antibody
    • CGI 44 antibody
    • CGI44 antibody
    • mitochondrial antibody
    • PRO1975 antibody
    • SQRD_HUMAN antibody
    • Sqrdl antibody
    • Sulfide dehydrogenase like antibody
    • Sulfide quinone reductase like antibody
    • Sulfide quinone reductase-like (yeast) antibody
    • Sulfide:quinone oxidoreductase antibody
    • Sulfide:quinone oxidoreductase, mitochondrial antibody
    see all

画像

  • ICC/IF image of ab71978 stained CACO-2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab71978 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-SQRDL antibody (ab71978) at 1/500 dilution

    Lane 1 : SQRDL transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

参考文献

ab71978 has not yet been referenced specifically in any publications.

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