The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Protein modification; protein ubiquitination.
Belongs to the SPSB family. Contains 1 B30.2/SPRY domain. Contains 1 SOCS box domain.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
Immunocytochemistry/Immunofluorescence analysis of Rhesus macaque spinal cord labeling SPSB2 using ab113778 at 1:300. An Alexa Fluor® 488-conjugated Donkey anti-rabbit (1:500) was used as the secondary antibody.
Western blot - SPSB2 antibody (ab113778)
Anti-SPSB2 antibody (ab113778) at 1 µg/ml + COLO205 cell lysate at 10 µg
Predicted band size : 29 kDa Gel concentration: 12%