The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. Can function as both amine donor and acceptor in transglutaminase-mediated cross-linkage.
Suprabasal layers of squamous-differentiated tissues such as epidermis, esophagus, tongue and trachea.
Belongs to the cornifin (SPRR) family.
Expressed during differentiation of squamous cells.
Immunohistochemistry (Frozen sections) analysis of mouse eyeball sections labelling SPRR1b with ab123237 at 1:50. An Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:50) was used as the secondary antibody.
Western blot - Anti-SPRR1b antibody (ab123237)
Anti-SPRR1b antibody (ab123237) at 1 µg/ml + Jurkat cell lysate at 10 µg
Secondary Goat anti-Rabbit IgG HRP
Predicted band size : 10 kDa Gel concentration: 10-20%.
Fang Z et al. Long non-coding RNA MALAT-1 modulates metastatic potential of tongue squamous cell carcinomas partially through the regulation of small proline rich proteins. BMC Cancer16:706 (2016).
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