Anti-SPRR1a 抗体 (ab125374)

Abreviews (1)Q&A (2)
Customer reviews and FAQs

製品の概要

  • 製品名Anti-SPRR1a antibody
  • 製品の詳細
    Rabbit polyclonal to SPRR1a
  • アプリケーション適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Cat, Pig
  • 免疫原

    Synthetic peptide corresponding to Human SPRR1a aa 40-89 (C terminal).
    Sequence:

    PCHPKVPEPCHPKVPEPCQPKVPEPCQPKVPEPCPSTVTPAPAQQKTKQK


    Database link: P35321

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • HT1080 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性The ability of neurons to regenerate an axon after injury is determined by both the surrounding environment and factors intrinsic to the damaged neuron. Neuronal SPRR1A is likely a significant contributor to successful nerve regeneration. The small proline-rich repeat protein 1A (SPRR1A) is not detectable in uninjured neurons but is induced by >60-fold after peripheral axonal damage being localized to injured neurons and axons. SPRR1A is one of a group of epithelial differentiation genes, including s100c and p21/waf, that are cocurrently induced in neurons by axotomy. Overexpressed SPRR1A colocalizes with F-actin in membrane ruffles and augments axonal outgrowth on a range of substrates. In axotomized sensory neurons, reduction of SPRR1A function restricts axonal outgrowth.
  • 細胞内局在Cytoplasmic
  • 参照データベース
    see all
  • 別名
    • 19 kDa pancornulin antibody
    • Cornifin A antibody
    • Cornifin alpha antibody
    • CornifinA antibody
    • Small proline rich protein 1A antibody
    • Small proline rich protein IA antibody
    • spr antibody
    • SPR IA antibody
    • SPRK antibody
    • SPRR 1a antibody
    • SPRR1A protein antibody
    see all

Anti-SPRR1a antibody 画像

  • Western blot - Anti-SPRR1a antibody (ab125374)
    Western blot - Anti-SPRR1a antibody (ab125374)
    Anti-SPRR1a antibody (ab125374) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Predicted band size : 10 kDa
  • Immunohistochemistry (Frozen sections) - Anti-SPRR1a antibody (ab125374)
    Immunohistochemistry (Frozen sections) - Anti-SPRR1a antibody (ab125374)This image is courtesy of an Abreview submitted by Yuan Cao
    ab125374 staining SPRR1a in Human skin tissue by Immunohistochemistry (Frozen sections).

    Tissue was fixed with acetone and blocked with 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/300 in 1% BSA + 10% goat serum) for 16 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    See Abreview

Anti-SPRR1a antibody (ab125374) 使用論文

ab125374 has not yet been referenced specifically in any publications.

ab125374 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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Immunohistochemistry (Frozen sections) abreview for Anti-SPRR1a antibody

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
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Yuan Cao

Verified customer

投稿 Jul 27 2012

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