Anti-SPRR1a 抗体 (ab125374)

製品の概要

  • 製品名Anti-SPRR1a antibody
  • 製品の詳細
    Rabbit polyclonal to SPRR1a
  • アプリケーション適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Cat, Pig
  • 免疫原

    Synthetic peptide corresponding to Human SPRR1a aa 40-89 (C terminal).
    Sequence:

    PCHPKVPEPCHPKVPEPCQPKVPEPCQPKVPEPCPSTVTPAPAQQKTKQK


    Database link: P35321

  • ポジティブ・コントロール
    • HT1080 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性The ability of neurons to regenerate an axon after injury is determined by both the surrounding environment and factors intrinsic to the damaged neuron. Neuronal SPRR1A is likely a significant contributor to successful nerve regeneration. The small proline-rich repeat protein 1A (SPRR1A) is not detectable in uninjured neurons but is induced by >60-fold after peripheral axonal damage being localized to injured neurons and axons. SPRR1A is one of a group of epithelial differentiation genes, including s100c and p21/waf, that are cocurrently induced in neurons by axotomy. Overexpressed SPRR1A colocalizes with F-actin in membrane ruffles and augments axonal outgrowth on a range of substrates. In axotomized sensory neurons, reduction of SPRR1A function restricts axonal outgrowth.
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • 19 kDa pancornulin antibody
    • Cornifin A antibody
    • Cornifin alpha antibody
    • CornifinA antibody
    • Small proline rich protein 1A antibody
    • Small proline rich protein IA antibody
    • spr antibody
    • SPR IA antibody
    • SPRK antibody
    • SPRR 1a antibody
    • SPRR1A protein antibody
    see all

Anti-SPRR1a antibody 画像

  • Anti-SPRR1a antibody (ab125374) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Predicted band size : 10 kDa
  • ab125374 staining SPRR1a in Human skin tissue by Immunohistochemistry (Frozen sections).

    Tissue was fixed with acetone and blocked with 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/300 in 1% BSA + 10% goat serum) for 16 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    See Abreview

Anti-SPRR1a antibody (ab125374) 使用論文

ab125374 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

yuan cao

Verified customer

投稿 Jul 27 2012

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I am very pleased to hear you would like to accept our offer and test ab125374 in IHC-P. This code will give you 1 free PRIMARY ANTIBODY before the e...

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