製品の概要
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製品名Anti-SPRR1a antibody
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製品の詳細Rabbit polyclonal to SPRR1a
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由来種Rabbit
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アプリケーション適用あり: IHC-Fr, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Cat, Pig
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免疫原
Synthetic peptide corresponding to Human SPRR1a aa 40-89 (C terminal).
Sequence:PCHPKVPEPCHPKVPEPCQPKVPEPCQPKVPEPCPSTVTPAPAQQKTKQK
Database link: P35321 -
ポジティブ･コントロール
- HT1080 cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーConstituents: 97% PBS, 2% Sucrose
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Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab125374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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関連性The ability of neurons to regenerate an axon after injury is determined by both the surrounding environment and factors intrinsic to the damaged neuron. Neuronal SPRR1A is likely a significant contributor to successful nerve regeneration. The small proline-rich repeat protein 1A (SPRR1A) is not detectable in uninjured neurons but is induced by >60-fold after peripheral axonal damage being localized to injured neurons and axons. SPRR1A is one of a group of epithelial differentiation genes, including s100c and p21/waf, that are cocurrently induced in neurons by axotomy. Overexpressed SPRR1A colocalizes with F-actin in membrane ruffles and augments axonal outgrowth on a range of substrates. In axotomized sensory neurons, reduction of SPRR1A function restricts axonal outgrowth.
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細胞内局在Cytoplasmic
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参照データベース
- Entrez Gene: 6698 Human
- Entrez Gene: 20753 Mouse
- Entrez Gene: 499660 Rat
- Omim: 182265 Human
- SwissProt: P35321 Human
- SwissProt: Q62266 Mouse
- SwissProt: Q63532 Rat
- Unigene: 46320 Human
see all -
別名
- 19 kDa pancornulin antibody
- Cornifin A antibody
- Cornifin alpha antibody
see all
画像
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Western blot - Anti-SPRR1a antibody (ab125374)Anti-SPRR1a antibody (ab125374) at 1 µg/ml + HT1080 cell lysate at 10 µg
Predicted band size: 10 kDa
Gel concentration: 10-20% -
Immunohistochemistry (Frozen sections) - Anti-SPRR1a antibody (ab125374)This image is courtesy of an Abreview submitted by Yuan Caoab125374 staining SPRR1a in Human skin tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed with acetone and blocked with 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/300 in 1% BSA + 10% goat serum) for 16 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).
参考文献
ab125374 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"