Anti-Sprouty 4 (phospho Y75) 抗体 (ab103114)

製品の概要

  • 製品名
    Anti-Sprouty 4 (phospho Y75) antibody
    Sprouty 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sprouty 4 (phospho Y75)
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 57-86 from Tyrosine 75 region of Human Sprouty 4

  • ポジティブ・コントロール
    • K-562 cell lysate. Human brain tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab103114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 32 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
  • 配列類似性
    Belongs to the sprouty family.
    Contains 1 SPR (sprouty) domain.
  • ドメイン
    The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
  • 細胞内局在
    Cytoplasm. Cell projection > ruffle membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Protein sprouty homolog 4 antibody
    • Sprouty homolog 4 antibody
    • sprouty homolog 4 (Drosophila) antibody
    • SPRY 4 antibody
    • Spry-4 antibody
    • Spry4 antibody
    • SPY4_HUMAN antibody
    see all

Anti-Sprouty 4 (phospho Y75) antibody 画像

  • ab103114, at 1/50 dilution, staining Sprouty 4 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
  • Anti-Sprouty 4 (phospho Y75) antibody (ab103114) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 32 kDa

Anti-Sprouty 4 (phospho Y75) antibody (ab103114) 使用論文

ab103114 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (MCF7)
Specification
MCF7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jul 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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