Rabbit polyclonal to Sprouty 4 (phospho Y75)
Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 57-86 from Tyrosine 75 region of Human Sprouty 4
K-562 cell lysate. Human brain tissue.
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
Purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 32 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
Belongs to the sprouty family.
Contains 1 SPR (sprouty) domain.
The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
Cytoplasm. Cell projection > ruffle membrane.
Information by UniProt
Protein sprouty homolog 4 antibody
Sprouty homolog 4 antibody
sprouty homolog 4 (Drosophila) antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sprouty 4 (phospho Y75) antibody (ab103114)
ab103114, at 1/50 dilution, staining Sprouty 4 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
Western blot - Anti-Sprouty 4 (phospho Y75) antibody (ab103114)
Anti-Sprouty 4 (phospho Y75) antibody (ab103114) at 1/100 dilution + K562 cell lysate at 35 µg
Predicted band size: 32 kDa
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