This fast track antibody is not yet fully characterized. It is subject to these terms and conditions



  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


  • 追加情報Tested in peptide ELISA.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    This antibody has been tested by Western blot against cell lysates. We believe that Sprouty 4 is represented by the doublet at ~ 35kDa. We believe that this protein may require IP before Western blotting, corresponding to results seen in Sasaki et al. We have not yet succeeded in peptide blocking the bands detected with this antibody.

  • ターゲット情報

    Anti-Sprouty 4 antibody 画像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Western blot using ab7513 at 1/500.
      Lane 1: Hela nuclear
      Lane 2: Hela
      Lane 3: A431
      Lane 4: Jurkat
      Lane 5: 293

      Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
      Exposure time: 2minutes.
      Lysates at 5µg per lane.

      We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD.

    • All lanes : Anti-Sprouty 4 antibody (ab7513) at 1/100 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : SW13 whole cell lysate
      Lane 3 : C2C12 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.
    • ab7513 at 2.5 µg/ml staining human heart tissue.

    • ab7513 staining tumour cells in human liver tissue.  Brown color indicates presence of protein, blue color shows cell nuclei.

    Anti-Sprouty 4 antibody (ab7513) 使用論文

    This product has been referenced in:
    • So WK  et al. Sprouty4 mediates amphiregulin-induced down-regulation of E-cadherin and cell invasion in human ovarian cancer cells. Tumour Biol 37:9197-207 (2016). Read more (PubMed: 26768617) »
    • Barbáchano A  et al. SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity. Oncogene 29:4800-13 (2010). WB ; Human . Read more (PubMed: 20543868) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Melanoma)
    Loading amount 500 µg
    Specification Melanoma
    Gel Running Conditions Reduced Denaturing (15 %)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    投稿 Sep 06 2010

    Thank you for your enquiry. Ab7513 is a Fast-Track antibody and has not yet been fully characterized. Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testi...

    Read More