製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Sprouty 4
  • 特異性
    This antibody reacts specifically with human 32 kDa Sprouty 4 protein.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chicken, Cow
  • 免疫原

    Synthetic peptide derived from N terminal region of human Sprouty 4.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: None
    Constituents: Whole serum
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 32 kDa.

ターゲット情報

プロトコール

Anti-Sprouty 4 antibody (ab59785) 使用論文

This product has been referenced in:
  • Edmonds MD  et al. MicroRNA-31 initiates lung tumorigenesis and promotes mutant KRAS-driven lung cancer. J Clin Invest 126:349-64 (2016). Read more (PubMed: 26657862) »
  • Lee JY  et al. Novel Function of Sprouty4 as a Regulator of Stemness and Differentiation of Embryonic Stem Cells. Dev Reprod 20:171-7 (2016). WB . Read more (PubMed: 27660833) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 4 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 4°C
Sample
Mouse Cell (8-cell stage mouse pre-implantation embryo)
Specification
8-cell stage mouse pre-implantation embryo
Permeabilization
Yes - 0.5% Triton X-100 in 1xPBS
Fixative
Paraformaldehyde
Username

Aleksandar Mihajlovic

Verified customer

投稿 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (skin wound)
Loading amount
15 µg
Specification
skin wound
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Precedia Massey

Verified customer

投稿 Feb 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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