Rabbit polyclonal to Sprouty 4
conjugated to KLH, corresponding to C terminal amino acids 306-322 of Human Sprouty 4 (NP_112226.2).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Sodium azide Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/3000 - 1/12000.
1/100 - 1/1000. Predicted molecular weight: 33 kDa.
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
Belongs to the sprouty family.
Contains 1 SPR (sprouty) domain.
The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
Cytoplasm. Cell projection > ruffle membrane.
Information by UniProt
Protein sprouty homolog 4 antibody
Sprouty homolog 4 antibody
sprouty homolog 4 (Drosophila) antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4 antibody (ab115557)
ab115557, at 2.5µg/ml, staining Sprouty 4 in Formalin-fixed, Paraffin-embedded Human heart tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
has not yet been referenced specifically in any publications.
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