製品の概要

  • 製品名Anti-Sprouty 1 antibody
    Sprouty 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sprouty 1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human, Zebrafish
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region of Human Sprouty 1.

  • ポジティブ・コントロール
    • HeLa cell lysate.
  • 特記事項The reactivity to rat seems to be batch dependent.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

ターゲット情報

Anti-Sprouty 1 antibody 画像

  • Anti-Sprouty 1 antibody (ab75492) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-Sprouty 1 antibody (ab75492) at 5 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish liver homogenate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

Anti-Sprouty 1 antibody (ab75492) 使用論文

This product has been referenced in:
  • Adam O  et al. Role of miR-21 in the pathogenesis of atrial fibrosis. Basic Res Cardiol 107:278 (2012). WB ; Rat . Read more (PubMed: 22760500) »
  • Barbáchano A  et al. SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity. Oncogene : (2010). WB ; Human . Read more (PubMed: 20543868) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (HEK293 cells transfected with plasmid expressing)
Loading amount 20 µg
Specification HEK293 cells transfected with plasmid expressing
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Feb 01 2012

Thank you very much for your answer. After looking through the information provided, I would like to point out the following: 1.) We do not have tested rat as a species, and obviously therefore rat is not a batch tested species neither....

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Thank you for contacting us. I am very sorry to hear you are experiencing difficulties with the new batches of this product. We take product complaints very seriously, and investigate every product and batch that we feel may not be performing correctly...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"