製品の概要

  • 製品名Anti-Sprouty 1 antibody
    Sprouty 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Sprouty 1
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-111 of Human Sprouty 1

  • ポジティブ・コントロール
    • HepG2 cell lysate, heart tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab56670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
  • 配列類似性Belongs to the sprouty family.
    Contains 1 SPR (sprouty) domain.
  • ドメインThe Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
  • 細胞内局在Cytoplasm. Membrane. Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hSPRY1 antibody
    • Protein sprouty homolog 1 antibody
    • Sprouty homolog 1 antagonist of FGF signaling antibody
    • Sprouty homolog 1 antibody
    • Spry-1 antibody
    • Spry1 antibody
    • SPY1_HUMAN antibody
    see all

Anti-Sprouty 1 antibody 画像

  • Overlay histogram showing HepG2 cells stained with ab56670 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56670, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Sprouty 1 antibody (ab56670) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart.


  • Predicted band size : 35 kDa
    Sprouty 1 antibody (ab56670) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

Anti-Sprouty 1 antibody (ab56670) 使用論文

ab56670 has not yet been referenced specifically in any publications.

Product Wall

I am very pleased to hear you would like to accept our offer and test these products in flow cytometry. Each code will give you the value listed off your next order before the expiration date. To redeem this offer, please submit an Abreview for flow cy...

Read More

Thank you very much for your interest our AbTrial program. To our knowledge, our Spouty 1 or Sprouty 2 antibodies have not been tested in flwo cytometry. However by participating in our AbTrial program you can now use our products in an untested app...

Read More

Merci de participer à notre offre de test avec le ab56670 à la place de l'ab73447. Voici le nouveau code pour tester ab56670 chez l'ovin.

Code de remise : xx
Date d’expiration : xx

Comme pour l'autre offre d...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"