製品の概要

  • 製品名Anti-Sprouty 1 antibody
    Sprouty 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sprouty 1
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Sprouty 1.

  • ポジティブ・コントロール
    • WB: Human liver and fetal liver tissue lysates. ICC/IF: methanol fixed HeLa cells. IHC-P: Human normal liver tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab111523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

ターゲット情報

  • 機能May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
  • 配列類似性Belongs to the sprouty family.
    Contains 1 SPR (sprouty) domain.
  • ドメインThe Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
  • 細胞内局在Cytoplasm. Membrane. Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hSPRY1 antibody
    • Protein sprouty homolog 1 antibody
    • Sprouty homolog 1 antagonist of FGF signaling antibody
    • Sprouty homolog 1 antibody
    • Spry-1 antibody
    • Spry1 antibody
    • SPY1_HUMAN antibody
    see all

Anti-Sprouty 1 antibody 画像

  • All lanes : Anti-Sprouty 1 antibody (ab111523) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Human) Tissue Lysate - fetal normal tissue (ab29890)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab111523 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab111523 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of Sprouty 1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab111523, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

Anti-Sprouty 1 antibody (ab111523) 使用論文

ab111523 has not yet been referenced specifically in any publications.

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