製品の概要

  • 製品名
    Anti-SPRED1 antibody [M23-P2G3]
    SPRED1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [M23-P2G3] to SPRED1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from within residues 400 - 500 of Human SPRED1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: HeLa whole cell, Jurkat whole cell, human brain tissue, mouse brain tissue, rat brain tissue
  • 特記事項

    This antibody clone is manufactured by Abcam.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

ターゲット情報

  • 機能
    Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow.
  • 組織特異性
    Weakly expressed in embryonic cell line (HEK-293).
  • 関連疾患
    Defects in SPRED1 are the cause of Legius syndrome (LEGIUSS) [MIM:611431]. It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.
  • 配列類似性
    Contains 1 KBD domain.
    Contains 1 SPR (sprouty) domain.
    Contains 1 WH1 domain.
  • 翻訳後修飾
    Phosphorylated on tyrosine.
  • 細胞内局在
    Cell membrane. Membrane > caveola. Nucleus. Localized in cholesterol-rich membrane raft/caveola fractions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EVH1 domain-containing protein 1 antibody
    • EVH1/Sprouty domain containing protein antibody
    • FLJ33903 antibody
    • hSpred 1 antibody
    • hSpred1 antibody
    • NFLS antibody
    • PPP1R147 antibody
    • protein phosphatase 1 regulatory subunit 147 antibody
    • SPRE1_HUMAN antibody
    • SPRED 1 antibody
    • Spred-1 antibody
    • spred1 antibody
    • Sprouty related EVH1 domain containing 1 antibody
    • sprouty related EVH1 domain containing protein 1 antibody
    • Sprouty related protein 1 with EVH 1 domain antibody
    • Sprouty-related antibody
    • Suppressor of Ras/MAPK activation antibody
    see all

画像

  • All lanes : Anti-SPRED1 antibody [M23-P2G3] (ab64740) at 10 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Brain (Rat) Tissue Lysate
    Lane 5 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 30 seconds

プロトコール

参考文献

This product has been referenced in:
  • Pasmant E  et al. SPRED1, a RAS MAPK pathway inhibitor that causes Legius syndrome, is a tumour suppressor downregulated in paediatric acute myeloblastic leukaemia. Oncogene N/A:N/A (2014). Read more (PubMed: 24469042) »

See 1 Publication for this product

レビューと Q&A

Thanks for your call today and for letting us know about the trouble with these antibodies. As we discussed, I am sending two free of charge replacements that should arrive tomorrow on the order ***. Please keep me updated on how these work in ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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