製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SPON1
  • アプリケーション
    適用あり: IHC-FoFr, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Rat SPON1.

    (Peptide available as ab41539.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Spinal Cord; Mouse Ovary; Mouse 14 day fetal lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/1000.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).

ターゲット情報

  • 関連性
    SPON1 is a member of a subgroup of the thrombospondin type 1 (TSR) class molecules, defined by two domains of homology, the FS1/FS2 and TSR domains. The TSRs of SPON1 proteins are typical of class 2 TSRs. SPON1, which is similar to thrombospondin, is a extracellular matrix attached molecule that promotes neurite outgrowth and inhibits angiogenesis. Analysis of gain and loss of function experiments reveal that SPON1 is required for accurate pathfinding of embryonic axons, and plays a dual role in patterning axonal trajectories. It promotes the outgrowth of commissural and inhibits the outgrowth of motor axons, and has also been implicated in inflammatory processes in the nervous system.
  • 細胞内局在
    Secreted protein; extracellular space; extracellular matrix.
  • 参照データベース
  • 別名
    • F spondin antibody
    • SPON 1 antibody
    • spondin 1 (f spondin) extracellular matrix protein antibody
    • Spondin 1 antibody
    • Spondin 1 extracellular matrix protein antibody
    • Spondin 1 precursor antibody
    • Spondin1 antibody
    • Vascular smooth muscle cell growth promoting factor antibody
    • VSGP antibody
    • VSGP/F spondin antibody
    see all

画像

  • IHC-FoFr image of SPON1 staining on Mouse Spinal Cord using ab40797 (1:1000).The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

  • ICC/IF image of ab40797 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40797, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Port SA  et al. The Oncogenic Fusion Proteins SET-Nup214 and Sequestosome-1 (SQSTM1)-Nup214 Form Dynamic Nuclear Bodies and Differentially Affect Nuclear Protein and Poly(A)+ RNA Export. J Biol Chem 291:23068-23083 (2016). Read more (PubMed: 27613868) »
  • Palmer GD  et al. F-spondin deficient mice have a high bone mass phenotype. PLoS One 9:e98388 (2014). IHC-P ; Mouse . Read more (PubMed: 24875054) »

See all 5 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Spinal cord)
Specification
Spinal cord
Username

Dr. Sophie Pezet

Verified customer

投稿 May 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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