Anti-SPOCD1 抗体 (ab122188)
Key features and details
- Rabbit polyclonal to SPOCD1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SPOCD1 antibody -
製品の詳細
Rabbit polyclonal to SPOCD1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
antigen sequence, corresponding to amino acids 995-1085 (SPLLSPGLEV THSSLLLAVL LPKEGLPDTA GSSPWLGKVQ KMVSFNSKVE KRYYQPDDRR PNVPLKGTPP PGGAWQQSQG RGSIAPRGIS A) of Human SPOCD1.
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ポジティブ・コントロール
- IHC-P: Human testis tissue; WB: U-251 MG and Human Plasma lysates; ICC/IF: A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122188の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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配列類似性
Contains 1 SPOC domain.
Contains 1 TFIIS central domain. - Information by UniProt
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参照データベース
- Entrez Gene: 90853 Human
- SwissProt: Q6ZMY3 Human
- Unigene: 62604 Human
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別名
- FLJ25348 antibody
- RP11-84A19.1 antibody
- SPOC domain-containing protein 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/immunofluorescence analysis of A431 cells labelling SPOCD1 with ab122188 shows positive staining in the nucleus and nucleoli.
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All lanes : Anti-SPOCD1 antibody (ab122188) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma (IgG/HSA depleted) lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab122188 は 2 報の論文で使用されています。
- Liang J et al. SPOCD1 promotes cell proliferation and inhibits cell apoptosis in human osteosarcoma. Mol Med Rep 17:3218-3225 (2018). PubMed: 29257309
- Wang Y et al. Differential expression profile analysis of lncRNA UCA1α regulated mRNAs in bladder cancer. J Cell Biochem 119:1841-1854 (2018). PubMed: 28815726