製品の概要

  • 製品名Anti-SPO11 antibody
    SPO11 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SPO11
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human SPO11 aa 108-157 (N terminal).
    Sequence:

    AQKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDI


    Database link: NP_036576

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab81695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (By similarity). Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2.
  • 組織特異性Highly expressed in testis.
  • 配列類似性Belongs to the TOP6A family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cancer/testis antigen 35 antibody
    • CT35 antibody
    • Meiotic recombination protein SPO 11 antibody
    • Meiotic recombination protein SPO11 antibody
    • MGC39953 antibody
    • SPATA43 antibody
    • Spermatogenesis associated 43 antibody
    • SPO 11 antibody
    • SPO11 antibody
    • SPO11 meiotic protein covalently bound to DSB antibody
    • SPO11 meiotic protein covalently bound to DSB homolog antibody
    • SPO11 meiotic protein covalently bound to DSB like antibody
    • SPO11_HUMAN antibody
    • TOPVIA antibody
    see all

Anti-SPO11 antibody 画像

  • Anti-SPO11 antibody (ab81695) (0.2-1 ug/ml) + HepG2 cell lysate

    Predicted band size : 44 kDa
  • Anti-SPO11 antibody (ab81695) at 1 µg/ml + human 293T

    Predicted band size : 44 kDa

Anti-SPO11 antibody (ab81695) 使用論文

This product has been referenced in:
  • Litvinov IV  et al. Ectopic expression of cancer-testis antigens in cutaneous T-cell lymphoma patients. Clin Cancer Res 20:3799-808 (2014). Human . Read more (PubMed: 24850846) »
  • Lindsey SF  et al. Potential role of meiosis proteins in melanoma chromosomal instability. J Skin Cancer 2013:190109 (2013). WB . Read more (PubMed: 23840955) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (Testis spreads-meiotic cells)
Specification Testis spreads-meiotic cells
Permeabilization Yes - Triton X 0.05%
Fixative Paraformaldehyde
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投稿 May 28 2013

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The product ab24355 is the SPO11 peptide that was used as an immunogen for the polyclonal antibody ab22508. This antibody is one of our Fast Track products, and has only been tested in ELISA against the immunizing...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"