Anti-Spinophilin/Neurabin 2 抗体 (ab18561)


  • 製品名Anti-Spinophilin/Neurabin 2 antibody
    Spinophilin/Neurabin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Spinophilin/Neurabin 2
  • 特異性Specific for rat spinophilin at 120kD. Also recognizes a band at ~95 kDa which is suspected to be a cleavage product of neurabin 2.
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human, Chimpanzee
  • 免疫原

    Fusion protein, corresponding to internal sequence amino acids 286-390 of Rat brain Neurabin 2.

  • ポジティブ・コントロール
    • Rat brain microsomal preparation.



Our Abpromise guarantee covers the use of ab18561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
  • 追加情報IP: 4 µg immunoprecipitates Neurabin 2 from 500 µg of rat brain microsomal preparation.
    WB: 0.25 - 1 µg/ml detected Neurabin 2 in 20 µg of a rat brain microsomal preparation. Detects a band of approximately 120 kDa (predicted molecular weight: 89 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). Required for hepatocyte growth factor (HGF)-induced cell migration.
    • 配列類似性Contains 1 PDZ (DHR) domain.
    • 翻訳後修飾Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 is mediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association with F-actin, but does not affect its binding to PP1.
    • 細胞内局在Cytoplasm > cytoskeleton. Nucleus. Cell projection > dendritic spine. Cell junction > synapse. Cell junction > adherens junction. Cytoplasm. Cell membrane. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > ruffle membrane. Enriched at synapse and cadherin-based cell-cell adhesion sites. In neurons, both cytosolic and membrane-associated, and highly enriched in the post-synaptic density apposed to exitatory synapses. Colocalizes with PPP1R2 at actin-rich adherens junctions in epithelial cells and in dendritic spines (By similarity). Accumulates in the lamellipodium, filopodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ30345 antibody
      • NEB2_HUMAN antibody
      • Neurabin II antibody
      • Neurabin-2 antibody
      • Neurabin-II antibody
      • Neurabin2 antibody
      • NeurabinII antibody
      • Neural tissue specific F actin binding protein II antibody
      • p130 antibody
      • PP1bp134 antibody
      • PPP1R6 antibody
      • PPP1R9 antibody
      • Ppp1r9b antibody
      • Protein phosphatase 1 regulatory subunit 9B antibody
      • SPINO antibody
      • Spinophilin antibody
      see all

    Anti-Spinophilin/Neurabin 2 antibody (ab18561) 使用論文

    This product has been referenced in:
    • Stankovic KM  et al. Gene expression profiling of nasal polyps associated with chronic sinusitis and aspirin-sensitive asthma. Laryngoscope 118:881-9 (2008). Read more (PubMed: 18391768) »

    See 1 Publication for this product

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Apteronotus leptorhynchus Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization Yes - 0.3% Triton X-100
    Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

    Abcam user community

    Verified customer

    投稿 Dec 03 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount 50 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (4-15 %)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    投稿 Nov 26 2012