製品の概要

  • 製品名Anti-Spindly antibody
    Spindly 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Spindly
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 475 and 525 of Human CCDC99 (NP_060255.2).

  • ポジティブ・コントロール
    • HeLa whole cell lysate or 293T cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab99344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 70 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
  • 配列類似性Belongs to the Spindly family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus. Cytoplasm > cytoskeleton > spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AA409762 antibody
    • Arsenite related gene 1 protein antibody
    • Arsenite-related gene 1 protein antibody
    • CCDC 99 antibody
    • Ccdc99 antibody
    • Coiled coil domain containing 99 antibody
    • Coiled-coil domain-containing protein 99 antibody
    • FLJ20364 antibody
    • FLJ40690 antibody
    • hSpindly antibody
    • MGC124977 antibody
    • Protein Spindly antibody
    • rhabdomyosarcoma antigen MU RMS 40.4A antibody
    • Rhabdomyosarcoma antigen MU-RMS-40.4A antibody
    • Rrhabdomyosarcoma antigen protein MU RMS 40.4A antibody
    • SPDL1 antibody
    • SPDLY_HUMAN antibody
    • Spindle apparatus coiled coil domain containing protein 1 antibody
    • Spindle apparatus coiled coil protein 1 antibody
    • Spindly antibody
    • Spindly homolog antibody
    see all

Anti-Spindly antibody 画像

  • All lanes : Anti-Spindly antibody (ab99344) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 70 kDa


    Exposure time : 3 minutes
  • 3µg ab99344 were used to precipitate CCDC99 from 1mg HeLa whole cell lysate.
    20% IP was loaded on gel and probed with ab99344 at 1µg/ml.
    Detection: chemiluminescence with exposure time of 10 seconds

Anti-Spindly antibody (ab99344) 使用論文

ab99344 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99344.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"